Homework Answers
Dilution is the one in which a unit volume of liquid of your interest is mixed with appropriate volume of a solvent to achieve the desired concentration. To calculate dilution factor (DF) we simply use the formula:
DF = Final volume/Aliquot volume
Here, aliquot volume is 100µl and final volume will be (100+450+450)= 1000µl so
DF = 1000/100 =10
A dilution factor of 10 means 1:10 dilution and to express it in percentage we will multiply it by 100 .
1/10=0.1 and multiplying it by 100 gives 10%
So, bacterial culture was diluted by a factor of 10 (10%).
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please help with whatever possible. thank you so much in advance. Name One use of serial...
please help with whatever possible. thank you so much in
advance.
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please help with whatever possible. thank you so much in
advance.
Name One use of serial dilutions is to calculate the concentration of microorganisms. Since it would usually be challenging or even impossible to actually count the number of microorganisms in a sample, the sample is diluted and plated to get a reasonable number of colonies to count (usually between 25 to 250 colonies is the goal). Since each colony on an agar plate theoretically grew from a single microorganism,...
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