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Calculate the restriction map of plasmid JLB51given the following restriction digest data. HindIII 3.82 0.18 kb...
7. Show the restriction map for pOA102, which is a total of 4.35 kb. Sal: 2.30, 0.25, 1.80 Hhaill...
7. Show the restriction map for pOA102, which is a total of 4.35 kb. Sal: 2.30, 0.25, 1.80 Hhaill: 210, 1.55, 0.70 Sall艹hall: 1.20. 1.10. 0.75 0.70, 0.35. 0.25 pDA102 5. Map the plasmid from the fragments shown below. Pstl: 4.36 kb EcoRI: 4.36 kb Sall: 4.36 kb EcoRI+Sall: 3.98 kb, 0.38 kb EcoRi +Pstl: 3.61 kb. 0.75 kb Psti Sall: 3.23 kb, 1.13 kb 6. Map pDA401 from the digests below (all given in kb units) Hindll: 3.82. 0.18...
When plasmid pD21 is digested with the following enzymes, these bands are produced (all in kb):...
When plasmid pD21 is digested with the following enzymes, these bands are produced (all in kb): HinIII : 3.82, 0.18 SaII : 2.35, 1.65 EcoRV : 3.0, 1.0 HindIII + SaII : 2.35, 1.20, 0.27, 0.18 HindIII + EcoRV : 1.87, 1.00, 0.95, 0.18 SaII + EcoRV : 1.60, 1.40, 0.75, 0.25 Draw a restriction map based on the fragments that were produced.
Restriction Mapping Below is a restriction map for the plasmid PGEN101 (total length - 20 kb)....
Restriction Mapping Below is a restriction map for the plasmid PGEN101 (total length - 20 kb). Using this map as a guide, give the number of restriction fragments along with their associated lengths that would result from digesting PGEN101 with the restriction enzymes EcoRI, BamHII, anda combination of EcoRI + BamHI. BamHI BamHI BamHI / PGEN101 (20 kb) Mb EcoRI Digest Performed Size Emments Obtained EcoRI........ BamHI.. EcoRI + BamHI.... Two freshmen college students, interested in becoming gene jocks, performed...
The table below presents the results of different digests of a plasmid. All sizes are in...
The table below presents the results of different digests of a plasmid. All sizes are in base pairs. Digest Size of Fragments HindIII 4.00 kb BamHI 2.35 kb, 1.65 kb EcoRI 4.00 kb, 2.50 kb,1.50 kb HindIII + BamHI 1.65 kb, 1.50 kb, 0.85 kb HindIII + EcoRI 3.50 kb, 3.00 kb, 2.50kb, 1.00 kb, 0.50 kb BamHI + EcoRI 2.00kb, 1.85kb, 1.65kb, 1.5kb, 0.35kb, 0.50 kb One of the enzymes used only partially digested the DNA. Which enzyme is...
You digest a 10Kb Linear ECoRI dna fragment with TWO restriction enzymes and obtain the following...
You digest a 10Kb Linear ECoRI dna fragment with TWO restriction enzymes and obtain the following data Hind iii..... 3kb, 7kb BamHi ... 2 kb and 8kb HInd iii + bamHI ..... 1kb, 2kb, 7kb Draw a restriction map of this DNA fragment, labeling the sites for EcoRI, bahHI, and HIndiii
I need help making a restriction digest map for both of these sets of fragments. Please...
I need help making a
restriction digest map for both of these sets of fragments. Please
explain the steps and include drawing.
Draw restriction maps for the plasmids that produced the following fragments from restriction digestion: Digest Performed Size of DNA fragments produced BamHI BamHI/HindI 2.5kb & 1.5kb BamHI/EcoRI 3kb & 1kb HindIlI/EcoRI 3.5kb &0.5kb 4kp Digest Performed Size of DNA fragments produced BamHI BamHI & Sal1 4kb, 5kb BamHI & Pst1 6kb& 3kb Sal1&Pst1 1kb& 8kb kb
B4. Answer ALL parts: The diagram below shows the restriction pattern of a plasmid cut with...
B4. Answer ALL parts: The diagram below shows the restriction pattern of a plasmid cut with the enzymes BamHI, EcoRI and Ndel. The digests are carried out with each enzyme alone and then with different combinations of the three enzymes. Enzyme Fragment sizes kb BamHI 1.4 10.6 EcoRI 4.5 7.5 Ndel 2.5 9.5 BamHI + EcoRI 45 3.4 1.4 BamHI + Ndel 1.4 2.5 5.9 EcoRI + Ndel 0.5 2.0 2.5 7.0 (a) Draw an agarose gel with restriction fragments...
Extra Credit (1 point all or nothing) A circular DNA plasmid was digested with the following...
Extra Credit (1 point all or nothing) A circular DNA plasmid was digested with the following results. Draw a map of the plasmid with all the restriction sites EcoRI: 7.0kb SalI: 7 kb HindIII: 4.0kb, 2.0kb, 1.0kbSalI + HindIII: 2.5kb, 2.0kb, 1.5kb, 1.0kb EcoRI + HindIII: 4.0kb, 2.0kb, 0.6kb, 0.4kb EcoRI + SalI: 2.9kb, 4.1kb
9. On Worksheet 16.IIIB is a restriction map of bacteriophage lambda. You digest some lambda DNA...
9. On Worksheet 16.IIIB is a restriction map of bacteriophage lambda. You digest some lambda DNA with the enzymes BamHI and HindIII separately and then load the fragments into an agarose gel and perform electrophoresis. Next, you perform a Southern analysis using the 4,878-bp EcoRI lambda fragment as a probe. a. Draw a picture of the electrophoresis gel, using the outline of the stained electrophoresis gel in Worksheet 16.IIIB (the two smallest HindIII fragments will run off the gel.) b....
Can someone please help me answer these? Neat work is greatly appreciated. Explanation will also be...
Can someone please help me answer these? Neat work is greatly
appreciated.
Explanation will also be appreciated (optional, if you have
time).
Thank you so much! I will give a big thumbs up :)
(1) You start with the pUC18 plasmid that is a -2.7 kb circular DNA molecule. The multiple cloning site (i.e., polylynker) has unique restriction sites in the following order: HindIII, BamHI, EcoRI. Recall that unique restriction site means that these sites are only found once on...
7. Show the restriction map for pOA102, which is a total of 4.35 kb. Sal: 2.30, 0.25, 1.80 Hhaill: 210, 1.55, 0.70 Sall艹hall: 1.20. 1.10. 0.75 0.70, 0.35. 0.25 pDA102 5. Map the plasmid from the fragments shown below. Pstl: 4.36 kb EcoRI: 4.36 kb Sall: 4.36 kb EcoRI+Sall: 3.98 kb, 0.38 kb EcoRi +Pstl: 3.61 kb. 0.75 kb Psti Sall: 3.23 kb, 1.13 kb 6. Map pDA401 from the digests below (all given in kb units) Hindll: 3.82. 0.18...
Restriction Mapping Below is a restriction map for the plasmid PGEN101 (total length - 20 kb). Using this map as a guide, give the number of restriction fragments along with their associated lengths that would result from digesting PGEN101 with the restriction enzymes EcoRI, BamHII, anda combination of EcoRI + BamHI. BamHI BamHI BamHI / PGEN101 (20 kb) Mb EcoRI Digest Performed Size Emments Obtained EcoRI........ BamHI.. EcoRI + BamHI.... Two freshmen college students, interested in becoming gene jocks, performed...
I need help making a
restriction digest map for both of these sets of fragments. Please
explain the steps and include drawing.
Draw restriction maps for the plasmids that produced the following fragments from restriction digestion: Digest Performed Size of DNA fragments produced BamHI BamHI/HindI 2.5kb & 1.5kb BamHI/EcoRI 3kb & 1kb HindIlI/EcoRI 3.5kb &0.5kb 4kp Digest Performed Size of DNA fragments produced BamHI BamHI & Sal1 4kb, 5kb BamHI & Pst1 6kb& 3kb Sal1&Pst1 1kb& 8kb kb
B4. Answer ALL parts: The diagram below shows the restriction pattern of a plasmid cut with the enzymes BamHI, EcoRI and Ndel. The digests are carried out with each enzyme alone and then with different combinations of the three enzymes. Enzyme Fragment sizes kb BamHI 1.4 10.6 EcoRI 4.5 7.5 Ndel 2.5 9.5 BamHI + EcoRI 45 3.4 1.4 BamHI + Ndel 1.4 2.5 5.9 EcoRI + Ndel 0.5 2.0 2.5 7.0 (a) Draw an agarose gel with restriction fragments...
9. On Worksheet 16.IIIB is a restriction map of bacteriophage lambda. You digest some lambda DNA with the enzymes BamHI and HindIII separately and then load the fragments into an agarose gel and perform electrophoresis. Next, you perform a Southern analysis using the 4,878-bp EcoRI lambda fragment as a probe. a. Draw a picture of the electrophoresis gel, using the outline of the stained electrophoresis gel in Worksheet 16.IIIB (the two smallest HindIII fragments will run off the gel.) b....
Can someone please help me answer these? Neat work is greatly
appreciated.
Explanation will also be appreciated (optional, if you have
time).
Thank you so much! I will give a big thumbs up :)
(1) You start with the pUC18 plasmid that is a -2.7 kb circular DNA molecule. The multiple cloning site (i.e., polylynker) has unique restriction sites in the following order: HindIII, BamHI, EcoRI. Recall that unique restriction site means that these sites are only found once on...
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