Extra Credit (1 point all or nothing) A circular DNA plasmid was digested with the following...
Extra Credit (1 point all or nothing)
A circular DNA plasmid was digested with the following results.
Draw a map of the plasmid with all the restriction sites EcoRI: 7.0kb SalI: 7 kb HindIII: 4.0kb, 2.0kb, 1.0kbSalI + HindIII: 2.5kb, 2.0kb, 1.5kb, 1.0kb EcoRI + HindIII: 4.0kb, 2.0kb, 0.6kb, 0.4kb EcoRI + SalI: 2.9kb, 4.1kb
Homework Answers
Based on the single restriction enzyme digestion, there is one EcoR1 and SalI site each and 3 Hind III sites that divide the 7kb plasmid into 3 fragments of 4,2 and 1kb length.
Each of the EcoR1 and SalI sites is placed in any one of these 3 fragments. By seeing the double digestions, we can place the sites and then the final EcoR1 and SalI digestion gives the placement of these shoes relative to reach other (2.5 + 0.4 = 2.9)
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Extra Credit (1 point all or nothing)
A circular DNA plasmid was digested with the following...
Calculate the restriction map of plasmid JLB51given the following restriction digest data. HindIII 3.82 0.18 kb...
Calculate the restriction map of plasmid JLB51given the following restriction digest data. HindIII 3.82 0.18 kb BamHI 2.35, 1.65 kb EcoRI 3.00, 1.0 kb HindIII, BamHI 2.35, 1.2, 0.27, 0.18 kb HindIII, EcoRI 1.87, 1.00, 0.95, 0.18 kb BamHI, EcoRI 1.6, 1.4, 0.75, 0.25 kb draw the circular map
Can someone please help me answer these? Neat work is greatly appreciated. Explanation will also be...
Can someone please help me answer these? Neat work is greatly
appreciated.
Explanation will also be appreciated (optional, if you have
time).
Thank you so much! I will give a big thumbs up :)
(1) You start with the pUC18 plasmid that is a -2.7 kb circular DNA molecule. The multiple cloning site (i.e., polylynker) has unique restriction sites in the following order: HindIII, BamHI, EcoRI. Recall that unique restriction site means that these sites are only found once on...
Now determine the sizes of the fragments generated after digesting the r-plasmid with each of the...
Now determine the sizes of the fragments generated after digesting the r-plasmid with each of the restriction enzymes or combination of restriction enzymes. r-plasmid digested with Pstl r-plasmid digested with EcoRI r-plasmid digested with both Pstl and EcoRI Question 8. (3 pts.) Use this information to determine the relative placement of these restriction sites in the r-plasmid, and then draw restriction site maps of the vector and the r-plasmid on the following circular chromosome templates. The map should show the...
When plasmid pD21 is digested with the following enzymes, these bands are produced (all in kb):...
When plasmid pD21 is digested with the following enzymes, these bands are produced (all in kb): HinIII : 3.82, 0.18 SaII : 2.35, 1.65 EcoRV : 3.0, 1.0 HindIII + SaII : 2.35, 1.20, 0.27, 0.18 HindIII + EcoRV : 1.87, 1.00, 0.95, 0.18 SaII + EcoRV : 1.60, 1.40, 0.75, 0.25 Draw a restriction map based on the fragments that were produced.
Restriction Mapping of a Linear DNA • 2 DNA is a linear and double-stranded DNA isolated...
Restriction Mapping of a Linear DNA • 2 DNA is a linear and double-stranded DNA isolated from bacteriophage lambda. This bacteriophage is a virus that infects E. coli and can undergo specialized transduction (see the transduction lab lecture). Assuming you obtained the following fragments from the restriction digestion. Draw the restriction map for the EcoRI and Hindi II enzymes in the 2. DNA. Fragment Size EcoRI 15300 6200 5250 2100 EcoRI + HindIII 15300 4400 4000 2100 1800 Hind!!! 17100...
III. Subclone the gene into plasmid, extract the plasmid DNA. 5. You know that your insert...
III. Subclone the gene into plasmid, extract the plasmid DNA. 5. You know that your insert (gene of interest, GOI) is flanked by the EcoRI sites, which makes this restriction enzyme a perfect candidate to cut out your gene. You also know that the GOI has a unique BamH1 restriction site. After subcloning the PCR product into the plasmid, a purified DNA preparation of the plasmid is digested to completion with BamHI restriction endonuclease. In separate reactions, the same preparation...
The table below presents the results of different digests of a plasmid. All sizes are in...
The table below presents the results of different digests of a plasmid. All sizes are in base pairs. Digest Size of Fragments HindIII 4.00 kb BamHI 2.35 kb, 1.65 kb EcoRI 4.00 kb, 2.50 kb,1.50 kb HindIII + BamHI 1.65 kb, 1.50 kb, 0.85 kb HindIII + EcoRI 3.50 kb, 3.00 kb, 2.50kb, 1.00 kb, 0.50 kb BamHI + EcoRI 2.00kb, 1.85kb, 1.65kb, 1.5kb, 0.35kb, 0.50 kb One of the enzymes used only partially digested the DNA. Which enzyme is...
You digest a 10Kb Linear ECoRI dna fragment with TWO restriction enzymes and obtain the following...
You digest a 10Kb Linear ECoRI dna fragment with TWO restriction enzymes and obtain the following data Hind iii..... 3kb, 7kb BamHi ... 2 kb and 8kb HInd iii + bamHI ..... 1kb, 2kb, 7kb Draw a restriction map of this DNA fragment, labeling the sites for EcoRI, bahHI, and HIndiii
Restriction Mapping Below is a restriction map for the plasmid PGEN101 (total length - 20 kb)....
Restriction Mapping Below is a restriction map for the plasmid PGEN101 (total length - 20 kb). Using this map as a guide, give the number of restriction fragments along with their associated lengths that would result from digesting PGEN101 with the restriction enzymes EcoRI, BamHII, anda combination of EcoRI + BamHI. BamHI BamHI BamHI / PGEN101 (20 kb) Mb EcoRI Digest Performed Size Emments Obtained EcoRI........ BamHI.. EcoRI + BamHI.... Two freshmen college students, interested in becoming gene jocks, performed...
An 9 kb circular plasmid is cut with the EcoRI restriction enzyme, and the reaction products...
An 9 kb circular plasmid is cut with the EcoRI restriction enzyme, and the reaction products are run on a DNA gel and stained with ethidium bromide. Bands of 1, 3 and 5 kb are seen. How many EcoRI sites are in the plasmid? Choose the one answer that is most correct. Group of answer choices: At least 2, At least 3, At least 5, None, At least 1, At least 4
Calculate the restriction map of plasmid JLB51given the following restriction digest data. HindIII 3.82 0.18 kb BamHI 2.35, 1.65 kb EcoRI 3.00, 1.0 kb HindIII, BamHI 2.35, 1.2, 0.27, 0.18 kb HindIII, EcoRI 1.87, 1.00, 0.95, 0.18 kb BamHI, EcoRI 1.6, 1.4, 0.75, 0.25 kb draw the circular map
Can someone please help me answer these? Neat work is greatly
appreciated.
Explanation will also be appreciated (optional, if you have
time).
Thank you so much! I will give a big thumbs up :)
(1) You start with the pUC18 plasmid that is a -2.7 kb circular DNA molecule. The multiple cloning site (i.e., polylynker) has unique restriction sites in the following order: HindIII, BamHI, EcoRI. Recall that unique restriction site means that these sites are only found once on...
Now determine the sizes of the fragments generated after digesting the r-plasmid with each of the restriction enzymes or combination of restriction enzymes. r-plasmid digested with Pstl r-plasmid digested with EcoRI r-plasmid digested with both Pstl and EcoRI Question 8. (3 pts.) Use this information to determine the relative placement of these restriction sites in the r-plasmid, and then draw restriction site maps of the vector and the r-plasmid on the following circular chromosome templates. The map should show the...
Restriction Mapping of a Linear DNA • 2 DNA is a linear and double-stranded DNA isolated from bacteriophage lambda. This bacteriophage is a virus that infects E. coli and can undergo specialized transduction (see the transduction lab lecture). Assuming you obtained the following fragments from the restriction digestion. Draw the restriction map for the EcoRI and Hindi II enzymes in the 2. DNA. Fragment Size EcoRI 15300 6200 5250 2100 EcoRI + HindIII 15300 4400 4000 2100 1800 Hind!!! 17100...
III. Subclone the gene into plasmid, extract the plasmid DNA. 5. You know that your insert (gene of interest, GOI) is flanked by the EcoRI sites, which makes this restriction enzyme a perfect candidate to cut out your gene. You also know that the GOI has a unique BamH1 restriction site. After subcloning the PCR product into the plasmid, a purified DNA preparation of the plasmid is digested to completion with BamHI restriction endonuclease. In separate reactions, the same preparation...
Restriction Mapping Below is a restriction map for the plasmid PGEN101 (total length - 20 kb). Using this map as a guide, give the number of restriction fragments along with their associated lengths that would result from digesting PGEN101 with the restriction enzymes EcoRI, BamHII, anda combination of EcoRI + BamHI. BamHI BamHI BamHI / PGEN101 (20 kb) Mb EcoRI Digest Performed Size Emments Obtained EcoRI........ BamHI.. EcoRI + BamHI.... Two freshmen college students, interested in becoming gene jocks, performed...
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