Homework Answers
ANSWER :
24. Option C
Restriction enzyme cut the DNA molecule at specific nucleotide sequence also called Restriction endonuclease or Molecular scissor. Restriction Enzyme cut the DNA at specific point is called Restriction site or target site.
25.Option B
Differentiated cell means the process in which a cell changes from one cell type to anather cell type.
Hematopoietic stem cells is the example of differentiated cell.This cell originate from bone marrow that gives to RBC,WBC,and platelets, it means Hematopoitic stem cell convert into above cell type.this cell also called adult stem cell which differenciate into other cell type.
Anather differentiated cell example is Mesechymal stem cells which is also from bone marrow that give rise to stromal cell , fat cells and types of bone cells that is osteoblast,osteoclasts,osteocytes.
26. Option A
Plasmid is the small circular DNA molecule used as a vector to transfer foreign DNA to host cell.
Plasmid have the capacity of self replicating ability that replicate itself in the host cell plasmid is a tool in biotechnology used to deliver the desired character to a reciepent cell. They are mainly found in bacteria but also exist naturally in archaea and eukaroytes like yeast and plants.
27.option B
Piece of DNA from two different sources are combined together called Recombinant DNA.or rDNA .The transgenic or genetically modified organism is with the help of this technique.
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24. What do restriction enzymes do? A Randomly cut DNA B. Produce protein C. Cut DNA...
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14. Restriction endonucleases are a. enzymes that restrict DNA synthesis b. enzymes that cut DNA in specific sequences c. nuclear proteins that are involved in transcription d. components of the ribosomes involved in protein synthesis 15. The first step in southern blotting is a. converting DNA into RNA b. cutting high molecular weight DNA into smaller pieces c. converting RNA into DNA d. radioactively labeling the DNA so it can be detected after the procedure is complete 16. The major...
1.) If we mix two restriction fragments cut with the same restriction enzyme in the presence of DNA ligase, ATP and appropriate buffer, we will obtain... A) protein B) carbohydrate C) recombinant DNA...
1.) If we mix two restriction fragments cut with the same restriction enzyme in the presence of DNA ligase, ATP and appropriate buffer, we will obtain... A) protein B) carbohydrate C) recombinant DNA or plasmid D) pAMP
1.When cloning a PCR product into a plasmid using restriction enzymes, the restriction enzyme recognition sequences...
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Which of the following is not a use of restriction enzymes? A. Bacteria produce them to...
Which of the following is not a use of restriction enzymes? A. Bacteria produce them to cut up foreign viral DNA B. Making recombinant DNA C. Cutting a vector when cloning a gene D. Replicating DNA during PCR
Which of the following makes restriction enzymes useful for DNA fingerprinting and RFLP analysis? a. they...
Which of the following makes restriction enzymes useful for DNA fingerprinting and RFLP analysis? a. they typically attach and ligate DNA fragments together b. they cause DNA to be translated into the plasmid c. they cause DNA to be transcribed into the plasmid d. they can only function when within the cytoplasm of a cell e. none of the others are true
Chromosomal and plasmid DNA can be cut into manageable pieces by restriction enzymes. Using agarose gel...
Chromosomal and plasmid DNA can be cut into manageable pieces by
restriction enzymes. Using agarose gel electrophoresis, the DNA
fragments can be separated on a gel, based on their lengths. In
order to see the fragments, a stain is typically added to the gel.
The size of each fragment can be determined by comparing each one
to a DNA molecular weight marker of known size.
Below is a map of pBR22 plasmid. The position and base pair
number of the...
Which is not true about restriction enzymes? a. They cut RNA b. They evolved as a...
Which is not true about restriction enzymes? a. They cut RNA b. They evolved as a bacteria defense mechanism c. They can leave single-stranded overhanging sequences called Sticky ends d. They recognize a specific target sequence e.They cut DNA
Restriction Enzymes are naturally found in bacteria that use them to A. Replicate DNA B. Correct...
Restriction Enzymes are naturally found in bacteria that use them to A. Replicate DNA B. Correct mutated DNA C. Cut up the DNA of invading Bacteriophage D. complete conjugation
46. What enzyme is used to make cDNA a. Ribozyme b. Reverse transcriptase C. Taq polymerase...
46. What enzyme is used to make cDNA a. Ribozyme b. Reverse transcriptase C. Taq polymerase d. Restriction endonuclease 47. Which of the following requires contact between a virus and a recipient bacterium for transfer of DNA? a. Crossing over b. Mutation c. Transduction d. Conjugation e. Transformation 48. In Recombinant DNA technology a vector is used to inserts the DNA into a host cell True/ False 49. If a foreign gene inserted into a plasmid inactivates the beta-galactosidase gene,...
LAB17 Genetic Engineering of Bacteria Problem Is it possible to transfer the allele for resistance to...
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Chromosomal and plasmid DNA can be cut into manageable pieces by
restriction enzymes. Using agarose gel electrophoresis, the DNA
fragments can be separated on a gel, based on their lengths. In
order to see the fragments, a stain is typically added to the gel.
The size of each fragment can be determined by comparing each one
to a DNA molecular weight marker of known size.
Below is a map of pBR22 plasmid. The position and base pair
number of the...
46. What enzyme is used to make cDNA a. Ribozyme b. Reverse transcriptase C. Taq polymerase d. Restriction endonuclease 47. Which of the following requires contact between a virus and a recipient bacterium for transfer of DNA? a. Crossing over b. Mutation c. Transduction d. Conjugation e. Transformation 48. In Recombinant DNA technology a vector is used to inserts the DNA into a host cell True/ False 49. If a foreign gene inserted into a plasmid inactivates the beta-galactosidase gene,...
LAB17 Genetic Engineering of Bacteria Problem Is it possible to transfer the allele for resistance to the antibiotic ampicillin into a bacterial cell? Objectives After completing this lab, the student will be able to: 1. Demonstrate micropipetting and sterile pipetting techniques for handling and transferring bacteria and plasmid DNA. 2. Maintain sterile conditions for culturing bacterial cells. 3. Inoculate bacteria into flasks, culture tubes, or agar plates. 4. Culture isolated individual colonies from an agar plate to form genetically identical...
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