Question

1. One enzyme will generally catalyze: a. Does not catalyze any reaction b. Only one unique reaction c. Several different reactions d. many different reactions e. none of the above 2. List three experimental conditions which would affect enzyme activity: (six different conditions were done in lab) 3. enzyme Reaction: ------> Ris called: Q is called: 4. One experimental method that would destroy enzyme activity:

Answer 1

Q1)

B. Only one unique reaction

Typically one enzyme can only catalyze one unique reaction. This is because of Substrate specificity, where a given enzyme can only fit one substrate in its catalytic cleft.

Q2)

Three experimental conditions which would affect enzymatic activity are:

1) Temperature: Enzymes functional optimally at a specific temperature. Changing the temperature drastically affects enzyme function.

2) pH: Like Temperature, Enzymes have an optimal pH at which they function. This is determined by the Amino acid composition of the protein.

3) Substrate Concentration: The substrate concentration in the solution also affects enzyme activity, with the Enzyme activity being highest at the saturating substrate concentration.

Q3)

R is the Product in the reaction, while Q is the Reactant or Substrate.

Q4)

One experimental method that would destroy enzymatic activity is boiling. Upon boiling the Enzyme solution, the protein conformational structure is destroyed which renders the Enzyme non-functional.