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You have the following mRNA sequence: 5’-GCAUGAUAUGCGAGCUAHCAUGACGU-3’ What is the DNA coding strand, template strand, and...

You have the following mRNA sequence: 5’-GCAUGAUAUGCGAGCUAHCAUGACGU-3’

What is the DNA coding strand, template strand, and tRNA sequence. Where is the start and stop codons and provide the resultant polypeptide sequence

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Answer #1

mRNA,

5’-GC AUG AUA UGC GAG CUA GCA UGA CGU-3'

(Start and stop codons are in bold)

Amino acid sequence (use genetic code),

N - met ile cys glu leu ala stop - C

Coding strand (similar to mRNA)

5' - GCATGATATGCGAGCTAGCATGACGT - 3'

Template strand (complementary to coding)

3' - CGTACTATACGCTCGATCGTACTGCA - 5'

tRNA (complementary to mRNA)

3' - CGUACUAUACGCUCGAUCGUACUGCA - 5'

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