Which of the following DNA samples has the highest melting temperature? DNA that is 40% C...

Question

Question

Which of the following DNA samples has the highest melting temperature?

DNA that is 40% C

DNA that is 30% T

DNA that is 30%G

DNA that is 40%A

Answer 1

Answer #1

a) DNA that is 40% C

According to Chargraff`s rule

(A) +(G) =(C)+(T)

(A) +(T)+(C)+(G) = 100%

As cytosine (C) pairs with Gaunine (G), therefore the proportional of gaunine should be equal to cytosine i,e

(C) =40%, (G) =40%,

Total GC content is 80%, and remaing 20% is AT content, it will have highest melting temperature by putting these values in following formula:

T_m = ( 4 x [G+C] ) + (2 x [A+T] ) ⁰C

Answer 2

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