Question

Which of the following DNA oligonucleotides has higher melting temperature? A) A-T-G-C-G-A-T-G-C T-A-C-G-C-T-A-C-G B) C-T-A-C-T-A-A-G-A G-A-T-G-A-T-T-C-T...

Which of the following DNA oligonucleotides has higher melting temperature?

A) A-T-G-C-G-A-T-G-C T-A-C-G-C-T-A-C-G

B) C-T-A-C-T-A-A-G-A G-A-T-G-A-T-T-C-T

C) T-A-C-G-C-T-A-T-A A-T-G-C-G-A-T-A-T

D) G-A-T-A-A-T-T-C-G C-T-A-T-T-A-A-G-C

The answer is A, can someone explain why?

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Answer #1

Answer A is correct because there is more Guanine Cytosine pairs which has three hydrogen bonds as compared to thymine adenine pairing which has two hydrogen bonds. Higher the GC content higher will be the melting point.

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Which of the following DNA oligonucleotides has higher melting temperature? A) A-T-G-C-G-A-T-G-C T-A-C-G-C-T-A-C-G B) C-T-A-C-T-A-A-G-A G-A-T-G-A-T-T-C-T...
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