Which of the following is a correct statement? Select one: a. If you want to make...
Which of the following is a correct statement?
Select one:
a. If you want to make a cDNA library, it doesn't matter which tissue you isolate the mRNA from.
b. If you want to make a genomic library, it doesn't matter which tissue you isolate the DNA from.
c. If you want to make a cDNA library, it doesn't matter which tissue you isolate the DNA from.
d. If you want to make a genomic library, it doesn't matter which tissue you isolate the mRNA from.
Homework Answers
"b. If you want to make a genomic library, it doesn't matter which tissue you isolate the DNA from" is correct statement.
cDNA library constructed with mRNA, and if the amount of mRNA isolated from a particular tissue is very low, we find diificulties to form cDNA library from it.
Genomic library formed from genomic DNA, and irrespective of amount of DNA isolated from any tissue. If the isolated DNA is very large, we digest it with restriction enzymes and if it is too small, we separate it with gel electrophoresis technique and then clone it with suitable vector.
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29. The central dogma of molecular genetics is that DNA encodes an mRNA, and mRNA allows proteins to be made. In the lecture on making cDNA libraries there was a statement that jokingly) said "central dogma be damned". What is it about making a cDNA library that goes against the central dogma? A. although a primer is required to make a cDNA, the primer is simply a long run of "T's", B....
I didnt do quite as well as i wpuld have liked on my molecular bio exam....
I
didnt do quite as well as i wpuld have liked on my molecular bio
exam. Please help me reciew what I missed. Thanks!
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29. The central dogma of molecular genetics is that DNA encodes an mRNA, and mRNA allows proteins to be made. In the lecture on making cDNA libraries there was a statement that jokingly) said "central dogma be damned". What is it about making a cDNA library that goes against the central dogma? A. although a primer is required to make a cDNA, the primer is simply a long run of "T's", B....
I
didnt do quite as well as i wpuld have liked on my molecular bio
exam. Please help me reciew what I missed. Thanks!
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