You want to know which areas of the genome are transcribed. Which of the following experiments...
You want to know which areas of the genome are transcribed. Which of the following experiments would give you the most complete answer of the research question?
| Make a genomic DNA library and sequence it |
| Make a cDNA library and sequence it |
| Hybridize a tilling array gene chip with the labeled RNA (or cDNA derived from RNA) extracted from the cell |
| Analyze the proteins expressed by the cell using mass spectrometry |
Homework Answers
The transcribed areas of genome can be found by hybridizing a tilling array gene chip with the labeled RNA (or cDNA derived from RNA) extracted from the cell. Option c is correct. The labelled RNA forms an hybrid molecule with transcriptable region of DNA and that can be isentified later.
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You want to know which areas of the genome are transcribed.
Which of the following experiments...
Which of the following is a correct statement? Select one: a. If you want to make...
Which of the following is a correct statement? Select one: a. If you want to make a cDNA library, it doesn't matter which tissue you isolate the mRNA from. b. If you want to make a genomic library, it doesn't matter which tissue you isolate the DNA from. c. If you want to make a cDNA library, it doesn't matter which tissue you isolate the DNA from. d. If you want to make a genomic library, it doesn't matter which...
54. The advantage of a cDNA library of eukaryotic genes compared with a genomic library is...
54. The advantage of a cDNA library of eukaryotic genes compared with a genomic library is that the cDNA library: a. always has the entire gene sequence b. lacks introns C. contains both introns and exons d. consists of single-stranded DNA e consists of RNA and DNA 55. Which of the following is an example of a cloning vector? a. Human growth hormone b. Mosquito c. Plasmid d. Tick e. Ribosomal RNA 56. Transformation in recombinant DNA technology: a. Requires...
I didnt do quite as well as i wpuld have liked on my molecular bio exam....
I
didnt do quite as well as i wpuld have liked on my molecular bio
exam. Please help me reciew what I missed. Thanks!
We were unable to transcribe this imageWe were unable to transcribe this imageA fragment restricted with EcoRI enzyme can be used for ligation into a plasmid that was restricted with BamHI because both the insert and the plasmid contain sticky ends a True b) False 10. Polynucleotide probes can be used to screen a genomic library...
Question 2 Imagine you are a scientist who intends to cure sickle cell disease. Which approach...
Question 2 Imagine you are a scientist who intends to cure sickle cell disease. Which approach would be best suited to this goal? a. Dietary supplements to make up for an enzyme deficiency b. Modification of ribosomes to enhance translation c. Exposure to X-rays to induce a new mutation d. Dietary supplements to make up for an amino acid deficiency e. Editing the gene for a polypeptide component of hemoglobin Question 7 Stop codons terminate translation by a. increasing peptidyl...
please help table 1 , Question 1 and 10 procedure is done which is the color...
please help
table 1 , Question 1 and 10
procedure is done which is the color result
taly Color in results from the slide microarray or attach a photo Label each gene appropriately O O o ооо Figure 1. Color Intensity chart Expression Ratios 16 1/4 1/8 1/16 Table 1 Gene 1 Gene 2 Gene 3 Gene 4 Gene 5 Gene 6 Expression Ratio Decimal Value Log2 Value Paper Microarray Exercise 1. Describe which genes were induced or repressed in...
molecular biology Section C (40 marks) Answer ALL questions from this Section 5. You have isolated total RNA from muscle cells and constructeda muscle cDNA library. You wish to study the regulatory r...
molecular biology
Section C (40 marks) Answer ALL questions from this Section 5. You have isolated total RNA from muscle cells and constructeda muscle cDNA library. You wish to study the regulatory region of a muscle-specific cDNA gene (gene M) that you have previously identified. 6 (a) For your study, you need to isolate a genomic clone of gene M. Why isa cDNA clone of gene M not appropriate for your study? (2 marks) (b) Outline the steps you would...
Which technique would you use if you wanted to < Identify all the regions of open...
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1. Which of the following RNAs can induce gene silencing? tRNA snRNA dsRNA rRNA miRNA 2....
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can you guys please give me the correct answers and explain why? 12. Which of the...
can you guys please give me the correct answers and explain
why?
12. Which of the following statements concerning histones is INCORRECT? A. The N-terminal tails of histone proteins are frequently post-translationally modified B. Histones are small, basic proteins. C. Histones bind DNA via hydrogen bonding with bases in the major groove. D. Histone HI is not one of the core histones. E. Histones are present in eukaryotes but not bacteria. V 13. You are studying two E coli genes...
54. The advantage of a cDNA library of eukaryotic genes compared with a genomic library is that the cDNA library: a. always has the entire gene sequence b. lacks introns C. contains both introns and exons d. consists of single-stranded DNA e consists of RNA and DNA 55. Which of the following is an example of a cloning vector? a. Human growth hormone b. Mosquito c. Plasmid d. Tick e. Ribosomal RNA 56. Transformation in recombinant DNA technology: a. Requires...
I
didnt do quite as well as i wpuld have liked on my molecular bio
exam. Please help me reciew what I missed. Thanks!
We were unable to transcribe this imageWe were unable to transcribe this imageA fragment restricted with EcoRI enzyme can be used for ligation into a plasmid that was restricted with BamHI because both the insert and the plasmid contain sticky ends a True b) False 10. Polynucleotide probes can be used to screen a genomic library...
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please help
table 1 , Question 1 and 10
procedure is done which is the color result
taly Color in results from the slide microarray or attach a photo Label each gene appropriately O O o ооо Figure 1. Color Intensity chart Expression Ratios 16 1/4 1/8 1/16 Table 1 Gene 1 Gene 2 Gene 3 Gene 4 Gene 5 Gene 6 Expression Ratio Decimal Value Log2 Value Paper Microarray Exercise 1. Describe which genes were induced or repressed in...
molecular biology
Section C (40 marks) Answer ALL questions from this Section 5. You have isolated total RNA from muscle cells and constructeda muscle cDNA library. You wish to study the regulatory region of a muscle-specific cDNA gene (gene M) that you have previously identified. 6 (a) For your study, you need to isolate a genomic clone of gene M. Why isa cDNA clone of gene M not appropriate for your study? (2 marks) (b) Outline the steps you would...
Which technique would you use if you wanted to < Identify all the regions of open chromatin in a particular cell type? Perform a paternity test? Amplify a specific sequence of DNA? [Choose] [Choose] Promoter fusion DNAse-seq Scanning mutagenesis Western blot RNAseq DNA fingerprinting Northern blot Reporter Assay DNAse footprinting ChIP-PCR EMSA Southern blot DNAse protection assay PCR BLAST search Protein fusion ChIP-seq [Choose Compare the global level of RNA expression between two samples? Detect presence of a specific protein...
The observation that in any DNA sample, A T and G C A. DNase sequencing An analytical method that determines which segments of DNA are bound by a particular B. Chargaff's rule protein factor, such as a transcription factor C. ChIP sequencing D. Euchromatin E. Histone acetylation F. major groove - # Areas associated with a eukaryotic gene that are where most DNA methylation occurs. # An analytical technique that involves a small slide or chip with many segments of...
can you guys please give me the correct answers and explain
why?
12. Which of the following statements concerning histones is INCORRECT? A. The N-terminal tails of histone proteins are frequently post-translationally modified B. Histones are small, basic proteins. C. Histones bind DNA via hydrogen bonding with bases in the major groove. D. Histone HI is not one of the core histones. E. Histones are present in eukaryotes but not bacteria. V 13. You are studying two E coli genes...
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