Which of the mechanisms involving siRNA- and miRNA-based gene regulation is INCORRECT? Select one: a. Cleavage of mRNA b. Inhibition of translation c. Post-translational modification d. Degradation of mRNA e. Transcriptional silencing
Which of the mechanisms involving siRNA- and miRNA-based gene regulation is INCORRECT? Select one: a. Cleavage...
Where does the following regulation happen? •transcriptional regulation •post-transcriptional regulation •post-translational regulation •miRNA/siRNA (mRNA degradation) •Ubiquitination/phosphorylation (protein degradation)
This short RNA (miRNA) is found in eukaryotic cells. miRNAs are post-transcriptional regulators that bind to complementary sequences on target mRNAs, resulting in enhancement of mRNA degradation by nucleases and/or _________. For the study of gene functions, artificial introduction of miRNA (siRNA) is performed to cells or animals. This experimental technique is called gene silencing and or _________, compared to the gene-deleting transgenic techniques. Select one: a. transcriptional repression ;;;; Knock-Down b. translational repression ;;;; Knock-Down c. transcriptional repression ;;;;...
Which of the mechanisms is not a mechanism of action of RNA inference? 1) inhibition of translation 2) transcriptional silencing 3) degradation of mRNA 4) ubiquitination of proteins 5) cleavage of mRNA It is NOT #2
Which of the following mechanisms to regulate gene expression are observed in eukaryotes, but are not generally observed in prokaryotes? Choose all that apply. Genes are grouped into operons, which allows transcriptional regulation to turn them all on together. RNA silencing selectively targets mRNA and destroys it to prevent further translation. Proteins can be activated or deactivated as a form of post-translational regulation. Epigenetic regulation can open or condense sectiosn of a chromosome, regulating which genes are transcribed. Regulatory proteins...
Which of the mechanisms is not a mechanism of action of RNA inference? 1) inhibition of translation 2) transcriptional silencing 3) degradation of mRNA 4) ubiquitination of proteins 5) cleavage of mRNA What is the basal transcription apparatus? 1)a complex of DNA polymerase, TATA boxes, and the DNA sequence 2) a complex of RNA polymerase, transcription factors, and other transcription proteins 3) a complex of DNA polymerase, transcription factors, and other transcription proteins 4) a complex of RNA polymerase, core...
Regulation of Gene Expression in Eukaryotes In eukaryotes, the Central Dogma of Genetics can be expanded to include post-transcriptional processing and post-translational processing, thus the Dogma becomes: Replication - Transcription Post-transcriptional Processing -Translation - Post-translational Processing - Functional Protein - Expressed Trait. This is the flow of information from the genetic material to the actual physical, chemical or behavioral trait in an organism. Of course, some traits are controlled by multiple genes and some also have an environmental component. At...
As indicated in the image below, miRNAs usually have imperfect base pairing with their target mRNAs, which leads to translational inhibition (the RISC complex is large, and won't let ribosomes through). On the other hand, siRNAs produced from longer double stranded RNA molecules (e.g. viruses), usually show perfect binding to their target mRNAs, which leads to mRNA degradation by the RISC complex. In 100 words or fewer explain why these two types of inhibitory ncRNAs differ in their mode of...
A gene-regulation strategy that is unique to eukaryotic cells is __________. See Section 19.1 ( page 380) . A. transcriptional regulation B. mRNA processing C. post-translational regulation D. Translational regulation
Which is the factor(s) involved in the regulation of gene activity? Select one: a. Transcriptional repressor/activator proteins, which bind to the operator/enhancer sequence regions. b. SAM binding to a riboswitch mRNA. c. Alterations in mRNA conformations by SAM during the transcription. d. All of these. e. None of these.
Identify mechanisms that can switch off gene expression. (Select all that apply) A. translation inhibition B. mRNA synthesis inhibition C. Methylation D. Phosphorylation E. Acetylation