Question

Given the following mRNA sequence, 5'–CGCAAGGCCUAU–3?', answer the questions below. A link to a table of codons can be found here.

Given the following mRNA sequence, 5'-CGCAAGGCCUAU-3', answer the questions below. A link to aa table of codons can be found here a) What amino acid sequence, using the three-letter designations for amino acids, is coded for by the mRNA? b) If a mutation converts AAG to CAG, what is the amino acid sequence? c) What will the amino acid sequence be if G is added to the beginning of the mRNA chain? Tools

Answer 1

(a) So to get the amino acid sequence for this problem, break the mRNA sequence into three letter chunks CGC-AAG-GCC-UAU Seconed Position code Amino Acid code Amino Acid code Amino Acid code Amino Acid UGU UGC UGA UGG CGU CGA ccc pro CAAn cGG UAU UCC UCA STOP STOP UAG STOP tp CUC leu CUA CUG AUU AUC AUA AUG GUU GUCval GUA CCA CCG ACU CAG AAU AGU AGC AGA AAAlys ACA ACG GCU GCC arg GGU GAC GAA GAG GGA glu GCG Using a genetic code table, you will use 3 consecutive bases to identify the amino acid. So CGC codes for Arg, AAG codes for Lys, GCC codes for Ala and UAU codes for Tyr 0, the amino acid sequence is Arg-Lys-Ala-Tyr

(b) If mutation converts AAG to CAG, the mRNA sequence in three letters is given as: CGC-CAG-GCC-UAU So, the amino acid sequence is: Arg-Gln-Ala-Tyr (c) Addition of G in the beginning of mRNA chain causes frame shift. GCG-CAA-GGC-CUA-U So, the amino acid sequence is: Ala-Gln-Gly-Leu