Question

The following sequence represents triplets on DNA

TAC CAG ATA CAC TCC CCT GCG ACT

A. Give the mRNA codons and tRNA anticodons that correspond with this sequence.

B. Using the above piece of DNA, show a deletion, insertion, a substitution, and nonsense mutation. Which ones are frame-shift mutations? Are any of your mutations missense? Use the table of codons and amino acids in the textbook to answer this part of the question.

For microbiology

Answer 1

triplets on DNA

TAC CAG ATA CAC TCC CCT GCG ACT

A. mRNA codons

AUG GUC UAU GUG AGG GGA CGC UGA

TRNA ANTICODONS

UAC CAG AUA CAC UCC CCU GCG ACU

B. A frameshift mutation is a genetic mutation caused by a deletion or insertion in a DNA sequence that shifts the way the sequence is read. A DNA sequence is a chain of many smaller molecules called nucleotides.

triplets on DNA

TAC CAG ATA CAC TCC CCT GCA CT

mRNA codons

AUG GUC UAU GUG AGG GGA CGU GA

Delettion of G occurs

triplets on DNA

TAC CAG ATA CAC TCC CCT GCG ACT

mRNA codons

AUG GUC UAU GUG AGG GGA CGC TAC T

Insertion of T occurs

NONSENSE MUTATION

triplets on DNA

TAC CAG ATA CAC TCC CCT GCG ACT

mRNA codons

AUG GUC UAU GUG AGG GGA CGC UGA

Nonsense mutation occurs

nonsense mutation, the new nucleotide changes a codon that specified an amino acid to one of the stop codons

triplets on DNA

TAC CAG ATA CAC TCC CCT GCG ACT

mRNA codons

AUG GUC UAU GUG AGG GGA CGC UGA

A changes to T

So mRNA

AUG GUC UAA

UAA is astop codon