You are given a sample of several compounds to separate by paper chromatography. You draw a...
You are given a sample of several compounds to separate by paper chromatography. You draw a pencil line exactly 1.0 cm from the bottom of the paper, and place a spot of sample on it. You dry the sample, then develop it in a solvent.
When the chromatogram is taken out of the solvent, the paper is wet up to 9.0 cm from the bottom of the sheet. The compound you are interested in shows up as a spot 7.3 cm from the bottom of the paper.
Calculate the following:
In the same time, how far did the solvent move?
Homework Answers
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You are given a sample of several compounds to separate by paper
chromatography. You draw a...
2. If two different compounds have the same Rr value, how might they be identified using paper chromatography?...
2. If two different compounds have the same Rr value, how might they be identified using paper chromatography? 3. Why is it important to keep the spots on the paper as small as possible? 4. If the solvent pool at the bottom of the beaker touched the spots on the 1.5cm line, what would happen to the spots? How would you address this error? 5. If you were teaching a student how to do paper chromatography, what three pieces of...
Question 5 (0.5 points) A certain compound is spotted on the starting line of a small paper chromatogram and that same compound is spotted again on a much larger paper chromatogram. Both papers...
Question 5 (0.5 points) A certain compound is spotted on the starting line of a small paper chromatogram and that same compound is spotted again on a much larger paper chromatogram. Both papers are developed using the same solvent and are pulled out when the solvent is 2 cm from the top. Which statement is true regarding the R of the spot after development? C) The spot on the larger paper should have a higher Rf because the spot travels...
Can someone please help me out with the Rf value PLEASE!! Thin Layer Chromatography Procedures 1)...
Can someone please help me out with the Rf value
PLEASE!!
Thin Layer Chromatography Procedures 1) Prepare a solvent solution in a 150 mL beaker by using 5 mL of Hexane and 3 mL of ethyl acetate Take one plate and spot (small spots) it with the three different standard solutions given. The standard solutions are 2-nitroaniline, 3-nitroaniline, and 4-nitroaniline. The fourth spot will be the unknown sample obtained. 2) 3) Align these spots at 1 cm from the bottom...
Case C Thin Layer Chromatography 1. TOXI-LAB is a screening method that uses: chromatography (column /...
Case C Thin Layer Chromatography 1. TOXI-LAB is a screening method that uses: chromatography (column / planar) The first step consists of the extraction of drug metabolites from biological fluids when we place the sample in a test tube containing a mixture of solvents and buffering salts that cause the extraction of basic and neutral drugs. The solvent extract of the sample is concentrated by heat and evaporation, then is deposited onto a small disc of chromatographic media (small circle...
Chromatography: Separating Mixtures Introduction: Magic marker inks are often mixtures of several compounds. Paper chromatography is...
Chromatography: Separating Mixtures Introduction: Magic marker inks are often mixtures of several compounds. Paper chromatography is a common method of separating various components of a mixture. After separation, you can observe the different colors that make up a particular color of magic marker ink. You can also calculate a ratio Rf, which compares how far each compound traveled to how far each solvent (substance that dissolves another substance) traveled during the experiment. Rf = Ds/Df Ds = Distance traveled by...
what is the prediction of the unknown amino acids mixture based on the strip from thin layer chromatography sheet? The unknown mixtures can be ala plus asp, ala plus asp plus lys, lys plus asp or ala...
what is the prediction of the unknown amino acids mixture
based on the strip from thin layer chromatography sheet?
The unknown mixtures can be ala plus asp, ala plus asp plus
lys, lys plus asp or ala plus lys.
anion exchange resin was used
Tubes 2,3, 6 and 7 had a spot appear that was light pink
We were unable to transcribe this imageWe were unable to transcribe this imageH-) to the column followed by a sufficient amount of the...
Place approximately 10 mg of each of the four reference compounds in labeled vials or 10 X 75-mm ...
can you solve this for me,
please
Place approximately 10 mg of each of the four reference compounds in labeled vials or 10 X 75-mm test tubes and dissolve the samples in a few drops of methanol. In a fifth tube place a quarter of an analgesic tablet and add 1 mL of methanol. Crush the tablet with a rod, stir well, and allow the insoluble material to settle. Note that aspirin is not stable in methanol, so fresh solutions...
this is regarding grignard reactions. thank you for any help given with how to go about...
this is regarding grignard reactions. thank you for any help given
with how to go about answering these questions !
1. Why is important that all glassware be dry? Write a mechanism showing why we cannot perform this reaction with wet glassware. 2. A mixture of the following compounds were run on a silica TLC using a mobile phase of diethylether and hexanes. Identify which spot is which and calculate the Revalues for each spot. 4.00 cm 3.80 cm ....
Table 2: Alcohol and Ketone Standards GC Retention Times GC#: Retention time Alcohol Standard Mixture Phent...
Table 2: Alcohol and Ketone Standards GC Retention Times GC#: Retention time Alcohol Standard Mixture Phent ion= 2.05Smin low Peak 1 Compound Name: athanol Rapesnal utanal aclapeatanal 4.755min oilng 6.895min .230 min Peak 2 Compound Name: Peak 3 Compound Name: Peak 4 Compound Name: GC#:1 Ketone Standard Mixture Retention time 2.575un Peak 1 Compound Name: Acclono Butanon a Pentanone 2tHex anon Peak 2 Compound Name: min Peak 3 Compound Name: 615 min 1D. 307 min Peak 4 Compound Name: Part...
-Give a brief background on the role of pigments in photosythensis. -state your hypothesis. -finish with...
-Give a brief background on the role of pigments in
photosythensis.
-state your hypothesis.
-finish with stating the rationale for your hypothesis (how
did you arrive at these hypothesis?)
this is an introduction piece, so it doesn't need the results to
write the papee. introduction sections set up the background,
purpose, rationale, and hypothesis for a study.
Biod 20 PHOTOSYNTHESIS Background Plants harness the energy of the sun through a process called photosynthesis. The leaf is the plant organ that...
2. If two different compounds have the same Rr value, how might they be identified using paper chromatography? 3. Why is it important to keep the spots on the paper as small as possible? 4. If the solvent pool at the bottom of the beaker touched the spots on the 1.5cm line, what would happen to the spots? How would you address this error? 5. If you were teaching a student how to do paper chromatography, what three pieces of...
Question 5 (0.5 points) A certain compound is spotted on the starting line of a small paper chromatogram and that same compound is spotted again on a much larger paper chromatogram. Both papers are developed using the same solvent and are pulled out when the solvent is 2 cm from the top. Which statement is true regarding the R of the spot after development? C) The spot on the larger paper should have a higher Rf because the spot travels...
Can someone please help me out with the Rf value
PLEASE!!
Thin Layer Chromatography Procedures 1) Prepare a solvent solution in a 150 mL beaker by using 5 mL of Hexane and 3 mL of ethyl acetate Take one plate and spot (small spots) it with the three different standard solutions given. The standard solutions are 2-nitroaniline, 3-nitroaniline, and 4-nitroaniline. The fourth spot will be the unknown sample obtained. 2) 3) Align these spots at 1 cm from the bottom...
Case C Thin Layer Chromatography 1. TOXI-LAB is a screening method that uses: chromatography (column / planar) The first step consists of the extraction of drug metabolites from biological fluids when we place the sample in a test tube containing a mixture of solvents and buffering salts that cause the extraction of basic and neutral drugs. The solvent extract of the sample is concentrated by heat and evaporation, then is deposited onto a small disc of chromatographic media (small circle...
what is the prediction of the unknown amino acids mixture
based on the strip from thin layer chromatography sheet?
The unknown mixtures can be ala plus asp, ala plus asp plus
lys, lys plus asp or ala plus lys.
anion exchange resin was used
Tubes 2,3, 6 and 7 had a spot appear that was light pink
We were unable to transcribe this imageWe were unable to transcribe this imageH-) to the column followed by a sufficient amount of the...
can you solve this for me,
please
Place approximately 10 mg of each of the four reference compounds in labeled vials or 10 X 75-mm test tubes and dissolve the samples in a few drops of methanol. In a fifth tube place a quarter of an analgesic tablet and add 1 mL of methanol. Crush the tablet with a rod, stir well, and allow the insoluble material to settle. Note that aspirin is not stable in methanol, so fresh solutions...
this is regarding grignard reactions. thank you for any help given
with how to go about answering these questions !
1. Why is important that all glassware be dry? Write a mechanism showing why we cannot perform this reaction with wet glassware. 2. A mixture of the following compounds were run on a silica TLC using a mobile phase of diethylether and hexanes. Identify which spot is which and calculate the Revalues for each spot. 4.00 cm 3.80 cm ....
Table 2: Alcohol and Ketone Standards GC Retention Times GC#: Retention time Alcohol Standard Mixture Phent ion= 2.05Smin low Peak 1 Compound Name: athanol Rapesnal utanal aclapeatanal 4.755min oilng 6.895min .230 min Peak 2 Compound Name: Peak 3 Compound Name: Peak 4 Compound Name: GC#:1 Ketone Standard Mixture Retention time 2.575un Peak 1 Compound Name: Acclono Butanon a Pentanone 2tHex anon Peak 2 Compound Name: min Peak 3 Compound Name: 615 min 1D. 307 min Peak 4 Compound Name: Part...
-Give a brief background on the role of pigments in
photosythensis.
-state your hypothesis.
-finish with stating the rationale for your hypothesis (how
did you arrive at these hypothesis?)
this is an introduction piece, so it doesn't need the results to
write the papee. introduction sections set up the background,
purpose, rationale, and hypothesis for a study.
Biod 20 PHOTOSYNTHESIS Background Plants harness the energy of the sun through a process called photosynthesis. The leaf is the plant organ that...
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