The answer is Lactose and glucose.
Kligler Iron Agar (KIA) is commonly used media to differentiate
the Enterobacteriaceae members by its capability of fementation of
lactose and glucose and hydrogen sulfide production. This KIA is
caleoritc method of differentiation. Based on the colors such as
yellow, red, black...etc around slant, butt are the main
differentitating characters to differentiate the Enterobacteriaceae
microbes.
Question 18 What two sugars must be present in Kligler's iron agar to yield the expected...
Following an 18-24 hour incubation period, what can you conclude from a TSI agar if the top is red and the butt is yellow? The inoculated bacterium can only ferment glucose The inoculated bacterium can only ferment sucrose The inoculated bacterium can only ferment lactose The inoculated bacterium is able to ferment all the sugars in the agar The inoculated bacterium cannot ferment
Background Background continued... Questions Experiment 2: TSI The triple sugar iron agar (TSI) can identify gram negative bacteria based on how they metabolize carbohydrates. It contains three carbohydrates-glucose, lactose and sucrose, in addition to sodium thiosulfate which some bacteria use in the production of hydrogen sulfide (H2S). It also contains iron in the ferrous form and phenol red as a pH indicator. TSI is poured into a tube when heated to a liquid form, and the tube is slanted as...
Q.8. . Tributyrin is a A) Amino acid B) A starch C) A triglyceride D) None are correct O. 9. . What color is a positive urease test ? A) yellow B) Bright pink D) green e bacterial exoenzymes are not correctly mached with their fiunction to degrade macromolecules? A) Amylase starch B) Cellulase cellulose C) Tryptophanase - tryptophan D) Lipase -casein Q.11. . Kligler's Iron agar media A) Is a multiple test medium B) It detects fermentation of glucose...
Question 18 Lactose intolerance is the inability to digest some of the sugars in milk and is associated with a deficiency in the enzyme lactase. Considering what you know about lactose, which following statements about lactase is true? The metabolites of lactase bind to lactose Lactase is necessary to hydrolyze the glycosidic bond between galactose and glucose Lactase is necessary to metabolize all oligosaccharides Lactase breaks the beta linkage between fructose and glucose Moving to another question will save this...
TSI Triple Sugar Iron Agar Data Sheet The enterics, specifically coliforms, will show what result in the TSI? What is reversion and what organisms showed reversion in the TSI? What does a black color in the butt of the slant indicate in the TSI? What is the pH indicator in the TSI and what is the purpose of the pH indicator? Do all organisms use the sugars in the TSI? What organisms do not utilize the sugars and what is...
can somrone help me write an introduction for each agar. thank you ? Introduction: Selective and differential media are used frequently in Microbiology Pathology Labs to identify a known group of bacteria from a complex mix of other bacteria. The medium is designed to select against as many of the background organisms as possible by using a medium in which they will not grow but the organisms under investigation can grow hence the "Selective" part of the name. Any assayable...
Eswrs Collece 15. The activity of the enzyme phenylalanine deaminase can be detected by using the follwing ragent A. 3% Hydrogen Peroxide B. 10% alpha Naphtol C. Nitrate reagents A and B D. Kovac's reagent E. None of the above Questions 16 through 20 refers to the cass Case Study: A fecal sample from a patient with diarrhea gave the following results: 16. Isolate produced colorless colonies after incubation on MacConkey agar. This resat means thar the siolated organism is...
help please What type(s) of bacteria are favored to grow on eosin methylene blue (EMB) agar? Select one: O a Gram positive bacteria that can undergo anaerobic respiration. O b. Gram negative bacteria that can ferment glucose and/ or lactose, O c. Only those bacilli that can utilize citrate as a main carbohydrate source. O d. All Bacteria would grow equally well A bacterial species grows on a MacConkey agar plate, and the colonies have dark red color with hazy...
Bacillus subtilis there was no experiment to do Give Slant/Butt colors: What sugars were fermented (choose one)? A. none Was there Gas produced? How could you tell? Give Letter Code: B. Glucose only C. Glucose, and sucrose and/or lactose Was there H2S produced? How could you tell? What two enzymes are involved in H2S production? (use the names from Leboffe and Pierce SIM Tube Did your organism convert tryptophan into indole? How could you tell? Was your organism motile? Did...
Lab question Table 5A-1 Data Table for Benedict's and Lugol's Tests Benedict's Test Lugol's Test Origia Final Color Color Before Boiling Tube After After 1. Water 2. Starch 3. Glucose elue owrm 5. Sucrose 6. Onion juiceGen Crenve 7. Potato slice l ecue | Green | WhLte一一1tra-areen 8. Milk Did the results for each test support your hypotheses? Did your results support your null hypothesis? Did the results for cach test agree with your predictions? Explain any discrepancies. PART 2-LUGOL'S...