Homework Answers
Ans) Only glucose
Only glucose is fermented by Bacillus subtilis.Bacillus subtilis can ferment more than one type of sugar, such as sucrose and dextrose, but cannot ferment other types of sugars, such as lactose.
Ans) Yes gas was produced because bubbles can be seen.
Ans) Yes H2S is produced becuase colour changes to black.
Ans) Cysteine desulfhydrase and 3 mercaptopyruvate sulfurtransferase are two key enzymes involved in releasing H2S.
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Give Slant/Butt colors: What sugars were fermented (choose one)? A. none Was there Gas produced? ...
Format 192.168.12 Tools Table Window Help o 5 0 Home Insert Draw Design Layout Times New...
Format 192.168.12 Tools Table Window Help o 5 0 Home Insert Draw Design Layout Times New R. 12 . A- A B TUXX google.cc Oxidation and Reduction Enzymes References Mailings Review View A A A TEE . . . .A .A . NYCD Paste A. . + . . Some media are designed to give multiple test results. Examples include: 1. K ler's Iron agar slant which determines Glucose and Lactose fermentations and production of Hydrogen sulfide 2. SIM which...
find the unknown bacteria based on this results? List of possible Bacteria • Selective Unknown Bacteria...
find the unknown bacteria based on this results?
List of possible Bacteria • Selective Unknown Bacteria # 4. and Differential Media results for 4 Test MSA PEA Gram (+) to Bacillus cereus (bacillus) do 2. Bacillus mepeterum (becillus) 3. bacillus subtilis (bacillus) Blood 1-Starch Results Interpretation Presumptive ID red frowth Orpinism does not ferment manitol. Nonpathogenic No reochrom (-) good growth oganism is not inhibitelje gram Phenylethyl alcohol complete olipeskon 14 red blood cells on o nomeync activity B hemolytic...
Background Background continued... Questions Experiment 2: TSI The triple sugar iron agar (TSI) can identify gram...
Background
Background continued...
Questions
Experiment 2: TSI The triple sugar iron agar (TSI) can identify gram negative bacteria based on how they metabolize carbohydrates. It contains three carbohydrates-glucose, lactose and sucrose, in addition to sodium thiosulfate which some bacteria use in the production of hydrogen sulfide (H2S). It also contains iron in the ferrous form and phenol red as a pH indicator. TSI is poured into a tube when heated to a liquid form, and the tube is slanted as...
Practice Questions 1. You were given a bacteria for unknown investigation. To begin with, you performed...
Practice Questions 1. You were given a bacteria for unknown investigation. To begin with, you performed a Methyl Red test after inoculating and incubating them for 48 hours. You obtained no color change after addition of Methyl Red. You proceed forward and performed Voges Proskauer test. For this particular test, you obtained a red color change after addition of Barritt solution A and B. From the result, what kind of inference can you draw from the 2 results obtained about...
D e eest. Results are determined by one of two ways: Ord O Directly observe First...
D e eest. Results are determined by one of two ways: Ord O Directly observe First perform a chemical test using media for results OR reagents. THEN observe media for results For example read through Physical Factors: Atmospheric Oxygen Requirements. (you do not do this test for your unknown). Pene 04. 1 pt. What is it you are testing for with this test? This should be a general answer, not a list of the five types of metabolic pathways. Omnou...
1. Someone new is preparing media for lab and adds buffers to the EMB agar that...
1. Someone new is preparing media for lab and adds buffers to the EMB agar that raise the pH to 7.8. The starting pH of EMB plates should be 6.9 – 7.3. No one is aware of the change. The EMB plates are inoculated for identification of unknown bacteria. After the appropriate incubation time, the results are interpreted. A. Discuss the consequences of the change in starting pH to the interpretation of the results. (The answer is not that no...
Puzzles, I need one or two word answer for each question. Thank you An acidic dye...
Puzzles, I need one or two word answer for each question. Thank you An acidic dye will give a ___________ stain. Dye in which the color portion resides in the positive ion. A bacterial cell wall containing only 2-3 layers of peptidoglycan and surrounded by an outer membrane composed of phospholipids, lipopolysaccharide, lipoprotein, and proteins. (2 words) During starch hydrolysis, starch is broken down to __________. Secreted by bacteria into the surrounding environment in order to break down larger nutrient...
Bio 121 I need to make (yeast fermentation) lab report. This is the lab experiment and...
Bio 121
I need to make (yeast fermentation) lab
report.
This is the lab experiment and results:
This is a guide to making the lab report:
General Biology BIO121 Yeast Fermentation Lab Introduction Organisms stay alive by the utilization of energy through metabolism. The energy acquiring pathways in photosynthesis convert radiant energy from the sun into the chemical bond energy of carbohydrates. This photosynthetic process is limited to the producers or autotrophs, which include plants, photosynthetic bacteria and some protists....
A The patient was a 32-year-old Haitian male referred to the hospital with a 3-week history...
A The patient was a 32-year-old Haitian male referred to the hospital with a 3-week history of fever, nausea, vomiting, and diarrhea. Four days after returning from Haiti, where he had participated at a funeral with unembalmed bodies, he developed a temperature of 39.50C, myalgias, constipation, and rectal pain. He was admitted to a hospital for an overnight stay and given LV. cefotaxime. He was discharged on oral cephalexin. His symptoms recurred 2 weeks later and his therapy was changed...
Citrate Utilization Test OBSERVATIONS AND INTERPRETATIONS 1 Using Table 5-10, page 340, as a guide, enter...
Citrate Utilization Test OBSERVATIONS AND INTERPRETATIONS 1 Using Table 5-10, page 340, as a guide, enter your results in the table below. Organism Color Result (+ or -) Interpretation Control QUESTIONS 1 Consider the uninoculated tube. a. Is it a positive or a negative control? b. What information is provided by the uninoculated control? Rus. ay Manry bacteria that are able to metabolize citrate (as seen in the citric acid cycle) produce negative e this test. Why? Be specific. ative...
Format 192.168.12 Tools Table Window Help o 5 0 Home Insert Draw Design Layout Times New R. 12 . A- A B TUXX google.cc Oxidation and Reduction Enzymes References Mailings Review View A A A TEE . . . .A .A . NYCD Paste A. . + . . Some media are designed to give multiple test results. Examples include: 1. K ler's Iron agar slant which determines Glucose and Lactose fermentations and production of Hydrogen sulfide 2. SIM which...
find the unknown bacteria based on this results?
List of possible Bacteria • Selective Unknown Bacteria # 4. and Differential Media results for 4 Test MSA PEA Gram (+) to Bacillus cereus (bacillus) do 2. Bacillus mepeterum (becillus) 3. bacillus subtilis (bacillus) Blood 1-Starch Results Interpretation Presumptive ID red frowth Orpinism does not ferment manitol. Nonpathogenic No reochrom (-) good growth oganism is not inhibitelje gram Phenylethyl alcohol complete olipeskon 14 red blood cells on o nomeync activity B hemolytic...
Background
Background continued...
Questions
Experiment 2: TSI The triple sugar iron agar (TSI) can identify gram negative bacteria based on how they metabolize carbohydrates. It contains three carbohydrates-glucose, lactose and sucrose, in addition to sodium thiosulfate which some bacteria use in the production of hydrogen sulfide (H2S). It also contains iron in the ferrous form and phenol red as a pH indicator. TSI is poured into a tube when heated to a liquid form, and the tube is slanted as...
D e eest. Results are determined by one of two ways: Ord O Directly observe First perform a chemical test using media for results OR reagents. THEN observe media for results For example read through Physical Factors: Atmospheric Oxygen Requirements. (you do not do this test for your unknown). Pene 04. 1 pt. What is it you are testing for with this test? This should be a general answer, not a list of the five types of metabolic pathways. Omnou...
Bio 121
I need to make (yeast fermentation) lab
report.
This is the lab experiment and results:
This is a guide to making the lab report:
General Biology BIO121 Yeast Fermentation Lab Introduction Organisms stay alive by the utilization of energy through metabolism. The energy acquiring pathways in photosynthesis convert radiant energy from the sun into the chemical bond energy of carbohydrates. This photosynthetic process is limited to the producers or autotrophs, which include plants, photosynthetic bacteria and some protists....
A The patient was a 32-year-old Haitian male referred to the hospital with a 3-week history of fever, nausea, vomiting, and diarrhea. Four days after returning from Haiti, where he had participated at a funeral with unembalmed bodies, he developed a temperature of 39.50C, myalgias, constipation, and rectal pain. He was admitted to a hospital for an overnight stay and given LV. cefotaxime. He was discharged on oral cephalexin. His symptoms recurred 2 weeks later and his therapy was changed...
Citrate Utilization Test OBSERVATIONS AND INTERPRETATIONS 1 Using Table 5-10, page 340, as a guide, enter your results in the table below. Organism Color Result (+ or -) Interpretation Control QUESTIONS 1 Consider the uninoculated tube. a. Is it a positive or a negative control? b. What information is provided by the uninoculated control? Rus. ay Manry bacteria that are able to metabolize citrate (as seen in the citric acid cycle) produce negative e this test. Why? Be specific. ative...
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