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What is DNA fingerprinting and discuss the procedure and processes it is based on (20)

What is DNA fingerprinting and discuss the procedure and processes it is based on (20)

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Answer #1

DNA Finger printing is the process of determining an Individuals DNA characteristics

It is based on the principle that:-

  • DNA sequence in all the humans is not the same
  • 90%of DNA is same 10% is different
  • This method is done by matching the uncommon sequence of humans with suspects unique sequence

Steps:-

  • Isolation of DNA from the blood,semen or vaginal fluid sample
  • PCR for DNA replication to occur to obtain multiple copies of DNA
  • Treatment of DNA with restriction enzymes
  • Gel electrophoresis that separates the DNA molecules according to the size
  • Chemical treatment to separate strands of DNA
  • Cross-linking of DNA single strand against nylon using uv light
  • Patterns of band appear on photographic film
  • Radio active DNA sequence seen
  • This is called DNA Finger printing
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