What is DNA fingerprinting and discuss the procedure and processes it is based on (20)
DNA Finger printing is the process of determining an Individuals DNA characteristics
It is based on the principle that:-
Steps:-
What is DNA fingerprinting and discuss the procedure and processes it is based on (20)
answer these questions regarding PCR and DNA "fingerprinting" 1. Explain the concept of DNA” fingerprinting”. What is the reason that it is referred to as fingerprinting? 2. Explain the concept of restriction enzymes and how they are used for DNA mapping. Discuss the concept of PCR as it applies to this process. 3. Explain what is occurring at each location along the thin wires at either end in the electrophoresis box. note that fine bubbles were occurring
DNA fingerprinting is based on regions of DNA that are variable between individuals. Many of these differences between individuals produce no change in phenotype. This type of mutation is a(n) O a) deletion Ob) insertion Oc) silent mutation. d) substitution mutation. Question 8 (2 points) The order of the base pairs in a DNA molecule a) differs from species to species. b) is identical in all organisms.
Based on the pattern of DNA fingerprinting, which suspect is very likely present at the crime scene? Describe the scientific evidence that supports your conclusion. (10 points)
What type of restriction enzymes should be used for methods such as DNA fingerprinting? a. all types b. type 1 c. type 3 d. type 2
NA fingerprinting uses a process called gel electrophoresis to separate the fragments of DNA. Once the DNA fragments are sorted, the pattern of bands can be analyzed. 1)Gel Electrophoresis Procedure The smaller DNA fragments start to move away from the wells and the larger DNA fragments remain closer to the wells. 2)An electric current is passed through the gel. 3) DNA fragments are treated with a dye. 4)A restriction endonuclease is added to the DNA. 5)Using micropipettes, the DNA samples...
4. What is DNA fingerprinting and explain how this could be used in court or on a TV show such as CSI? 5. List the four "large molecules of life.” Identify their composition and structure, and describe one function they perform in the cell. 6. What is meant by the terms dominant and recessive alleles?
t Restriction enzymes can be useful for the following Biotech processes (check ALL that apply): DNA Replication DNA Fingerprinting Genetic Engineering Insulin production using E.coli Polymerase chain reaction
is DNA fingerprinting better at showing that two DNA samples came from the same person, from different people, or equally good at both. Support your reasoning
SHORT ANSWER 1. Explain how the technique of DNA fingerprinting works. 2. What is the purpose of using a restriction enzymes? 3. What is the technique of gel electrophoresis used for? 4. What is the overall charge on DNA and what gives DNA this charge? 5. If both the parents are carriers of sickle cell disease, what is the probability of having a child that has sickle cell disease? Draw a punnett square to determine the probabilty.
The use of DNA fingerprinting relies on the fact that A. most genes used for identification are dominant. B. no two people, except identical twins, have exactly the same DNA. C. the most important genes are different among most people. D. DNA fingerprints from different people can look very similar.