Homework Answers
Assumption: G+C=50 %, G+C=A+T and random distribution of bases,
MspI = C/CGG= 44 = 256bps
Hinf I = G/ANTC = 44 (for GATC) X 11(for N) = 256 bps
Sfi I = GGCCNNNN/NGGCC = 48 (for GGCC, GGCC) X 11 (for NNNNN) = 65536 bps
Psi I = TTA/TAA = 46 = 4096 bps
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15. A new bacterial species was found and its DNA was analysed. It has a 50%...
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Suppose you digest the genomic DNA of a particular organism with
the restriction enzyme SauA. Then you ligate the resulting fragment
into a unique BamHI cloning site of a plasmid. The sequence of the
restriction sites and position of cleavage is shown below
Note: X and Y and their complementary bases Z and Y’
respectively can be any base (A,C, G, or T)
1) As you can see, ligation is possible because the two restriction
enzymes produce compatible sticky ends....
can someone explain throughly on how to find a-c??? thanks!!! The following question will provide practice in interpreting and analyzing gel results. 5. You obtained the DNA electrophoresis gel be...
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someone explain throughly on how to find a-c??? thanks!!!
The following question will provide practice in interpreting and analyzing gel results. 5. You obtained the DNA electrophoresis gel below. Three samples of lambda phage DNA were digested with 3 different restriction enzymes and the digested DNA was applied to the gel in lane 4 and the bands were visualized. The Hind Ill digest was used as a molecular weight standard marker and produced 6 DNA fragments of known size:...
A linear piece of DNA has the following restrictions sites: You decided to set up an...
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You decided to set up an experiment where you added one of these
restriction enzymes to this DNA. After this DNA was digested with
that restriction enzyme, you separated the resulting fragment(s)
using agarose electrophoresis. After the gel was stained with
ethidium bromide, you observed the following gel (the DNA ladder is
your reference standard and is comprised of a series of DNA
fragments of known length).
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i need from a to i please step by step Question 2 (50 points) The template...
i need from a to i please step by step
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colony which one express gene Only correct explanation not just answears pls. how to determine correct...
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how to determine correct orientation by electrophoresis
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Suppose you digest the genomic DNA of a particular organism with
the restriction enzyme SauA. Then you ligate the resulting fragment
into a unique BamHI cloning site of a plasmid. The sequence of the
restriction sites and position of cleavage is shown below
Note: X and Y and their complementary bases Z and Y’
respectively can be any base (A,C, G, or T)
1) As you can see, ligation is possible because the two restriction
enzymes produce compatible sticky ends....
can
someone explain throughly on how to find a-c??? thanks!!!
The following question will provide practice in interpreting and analyzing gel results. 5. You obtained the DNA electrophoresis gel below. Three samples of lambda phage DNA were digested with 3 different restriction enzymes and the digested DNA was applied to the gel in lane 4 and the bands were visualized. The Hind Ill digest was used as a molecular weight standard marker and produced 6 DNA fragments of known size:...
A linear piece of DNA has the following restrictions sites:
You decided to set up an experiment where you added one of these
restriction enzymes to this DNA. After this DNA was digested with
that restriction enzyme, you separated the resulting fragment(s)
using agarose electrophoresis. After the gel was stained with
ethidium bromide, you observed the following gel (the DNA ladder is
your reference standard and is comprised of a series of DNA
fragments of known length).
a) Which restriction...
A linear piece of DNA has the following restrictions sites: Xbal Noti Psti EcoRi 2 kb 4 kb -> 1 kb 2 kb 3 kb You decided to set up an experiment where you added one of these restriction enzymes to this DNA. After this DNA was digested with that restriction enzyme, you separated the resulting fragment(s) using agarose electrophoresis. After the gel was stained with ethidium bromide, you observed the following gel (the DNA ladder is your reference standard...
i need from a to i please step by step
Question 2 (50 points) The template strand of a buman DNA and a plasmid are given below. The strands are read from left to right. Both DNAs are to be digested by the four base pair cutter restriction enzymes shown in the table Bacteria DNA: 3-ACAGACCATGGAGGCTCCCATGGTTAGCCTGAAGCTTCGA- 3'--CTTIGTGTOCCACCATGGCAGGTACCGAACCTCAACTTTCCTC.-5, Human DNA: Restriction Enzyme Name Ai Bi Ci Di Ei Sequence and type of eut GATC- 3 CTAG 5 T CGA-3 5- G...
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The bacterium Thermus thermophilus was first isolated from hot springs in Japan, living in an environment with an average temperature of 68 degree C (154 degree F). This temperature would kill you, but these organisms are adapted to it. One of the adaptations to this lifestyle includes DNA that is 70% G-C base pairs, one of the more extreme departures from the average 50%. The chromosome is about 2.13 times 10^6 bp in length, quite normal for a bacterial species....
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