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replication for each Hfr strain on the inside of the map to indicate polarity of the...
Suppose a researcher conducts matings between three Hfr bacterial strains and an F strain. The table...
Suppose a researcher conducts matings between three Hfr bacterial strains and an F strain. The table shows the order in which genes are incorporated for each strain. Determine the gene order of the chromosome by placing each gene on the outside of the circular chromosome map. Identify the origin of replication for each Hfr strain on the inside of the map to indicate polarity of the origin. Hfr strain Order of gene transfer ikvqw izrbm vkimb 3 Answer Bank Answer...
Please answer the following question. CIrcle the final answer. Thanks in advance. Suppose a researcher conducts...
Please answer the following question. CIrcle the final answer.
Thanks in advance.
Suppose a researcher conducts matings between three Hfr bacterial strains and an F strain. The table below shows the order in which genes are incorporated for each strain. Determine the gene order of the chromosome by placing each gene on the outside of the circular chromosome map. Label the origin of replication for each Hfr strain on the inside of the map to indicate polarity of the origin...
You use à new strain of Hfr E. coli to map several genes by using interrupted...
You use à new strain of Hfr E. coli to map several genes by using interrupted conjugation. You mix Hfr cells that are xy/+ mil+ mal+ met+ and sensitive to phage T6. You mix those cells with an F- strain that is xyl- mil- mal- met- and resistant to phage T6 to allow conjugation. At regular intervals, you remove samples and interrupt conjugation. You kill the Hfr cells in each sample by using the T6 phage and plate what's left...
7. Five Hfr strains A through E are derived from a single F strain of E....
7. Five Hfr strains A through E are derived from a single F strain of E. coli. The following chart shows the entry times in minutes of the first five markers into an F strain when each Hfr strain is used in an interrupted-conjugation experiment: A B C E pro gal mal strs ser ade his ade This gal pro mer 11 16 36 51 13 28 38 44 70 prot met xyl mal" strs 29 32 37 his 10...
An Hfr strain is used to map four genes in an interrupted mating experiment. The cross...
An Hfr strain is used to map four genes in an interrupted mating experiment. The cross is Hfr/a+b+c+ rifs ×F−a−b−c− rifr . (No map order is implied in the listing of the alleles; rifr is resistance to the antibiotic rifampicin.) The a+gene is required for the biosynthesis of nutrient A, the b+ gene for nutrient B, c+ for nutrient C. The cross is initiated at time = 0, and at various times, the mating mixture is plated on medium. Each plate contains minimal medium (MM) plus rifampicin....
This is a Hfr mapping question and I'm completely lost here Basically the bonus is a...
This is a Hfr mapping question and I'm completely lost
here
Basically the bonus is a puzzle. It is based on mapping done with 4 different Hfr strains. I've given you the minutes that that it takes for several of the markers on the map to transfer in as determined by Hfr mapping. All you have to do is put them in the correct order around the circular chromosome and show me how many minutes are in between each marker...
Four Hfr strains (1, 2, 3, 4) were isolated from the same prototrophic, strF strain. In...
Four Hfr strains (1, 2, 3, 4) were isolated from the same prototrophic, strF strain. In separate experiments using the technique of interrupted mating, these four Hfr strains donated the following markers to an auxotrophic, str F strain in the order shown: (only the first six genes transmitted were scored for each strain) Hfr Strain 1 Hfr Strain 2 Hfr Strain 3 Hfr Strain 4 A. What is the order of these markers on the circular chromosome of the original...
33. In E. coli, a streptomycin-sensitive, prototrophic Hfr strain was mated to a streptomycin-resistant F-strain auxotrophic...
33. In E. coli, a streptomycin-sensitive, prototrophic Hfr strain was mated to a streptomycin-resistant F-strain auxotrophic for a number of nutritional requirements. After conjugation for various times, the results shown at right were obtained. Of the four genes shown, which two genes map the closest to each other? azir tonr Percent of recipients receiving a given gene lact gal + 10 50 60 20 30 40 Time (minutes)
Please explain step by step... thank you! In order to determine the overall chromosome map for...
Please explain step by step... thank you!
In order to determine the overall chromosome map for E coli, three Hfr strains were obtained from a protrophic but erythromycin resistant F+ ancestor. The start times of transfer during conjugation matings with F- strains carrying auxotrophic, fermentation, and erythromycin- sensitivity markers were determined for each Hfr strain, as listed. A. Using the times for transfer of each marker, draw a unified chromosome map of E coli, showing the order and relative distances...
Three Hfr strains from the same bacterial species are analyzed for their ability to transfer genes...
Three Hfr strains from the same bacterial species are analyzed for their ability to transfer genes to F recipient bacteria. The data shown below list the origin of transfer (oriT) for each strain and give the order of genes, with the first gene on the left and the last gene on the right. Hfr Strain Genes Transferred Hfr 1 Hfr 2 oriT met pro leu gal oriT ala lac thr azi oriT azi trp met pro Hfr 3 Use the...
Suppose a researcher conducts matings between three Hfr bacterial strains and an F strain. The table shows the order in which genes are incorporated for each strain. Determine the gene order of the chromosome by placing each gene on the outside of the circular chromosome map. Identify the origin of replication for each Hfr strain on the inside of the map to indicate polarity of the origin. Hfr strain Order of gene transfer ikvqw izrbm vkimb 3 Answer Bank Answer...
Please answer the following question. CIrcle the final answer.
Thanks in advance.
Suppose a researcher conducts matings between three Hfr bacterial strains and an F strain. The table below shows the order in which genes are incorporated for each strain. Determine the gene order of the chromosome by placing each gene on the outside of the circular chromosome map. Label the origin of replication for each Hfr strain on the inside of the map to indicate polarity of the origin...
You use à new strain of Hfr E. coli to map several genes by using interrupted conjugation. You mix Hfr cells that are xy/+ mil+ mal+ met+ and sensitive to phage T6. You mix those cells with an F- strain that is xyl- mil- mal- met- and resistant to phage T6 to allow conjugation. At regular intervals, you remove samples and interrupt conjugation. You kill the Hfr cells in each sample by using the T6 phage and plate what's left...
7. Five Hfr strains A through E are derived from a single F strain of E. coli. The following chart shows the entry times in minutes of the first five markers into an F strain when each Hfr strain is used in an interrupted-conjugation experiment: A B C E pro gal mal strs ser ade his ade This gal pro mer 11 16 36 51 13 28 38 44 70 prot met xyl mal" strs 29 32 37 his 10...
This is a Hfr mapping question and I'm completely lost
here
Basically the bonus is a puzzle. It is based on mapping done with 4 different Hfr strains. I've given you the minutes that that it takes for several of the markers on the map to transfer in as determined by Hfr mapping. All you have to do is put them in the correct order around the circular chromosome and show me how many minutes are in between each marker...
Four Hfr strains (1, 2, 3, 4) were isolated from the same prototrophic, strF strain. In separate experiments using the technique of interrupted mating, these four Hfr strains donated the following markers to an auxotrophic, str F strain in the order shown: (only the first six genes transmitted were scored for each strain) Hfr Strain 1 Hfr Strain 2 Hfr Strain 3 Hfr Strain 4 A. What is the order of these markers on the circular chromosome of the original...
33. In E. coli, a streptomycin-sensitive, prototrophic Hfr strain was mated to a streptomycin-resistant F-strain auxotrophic for a number of nutritional requirements. After conjugation for various times, the results shown at right were obtained. Of the four genes shown, which two genes map the closest to each other? azir tonr Percent of recipients receiving a given gene lact gal + 10 50 60 20 30 40 Time (minutes)
Please explain step by step... thank you!
In order to determine the overall chromosome map for E coli, three Hfr strains were obtained from a protrophic but erythromycin resistant F+ ancestor. The start times of transfer during conjugation matings with F- strains carrying auxotrophic, fermentation, and erythromycin- sensitivity markers were determined for each Hfr strain, as listed. A. Using the times for transfer of each marker, draw a unified chromosome map of E coli, showing the order and relative distances...
Three Hfr strains from the same bacterial species are analyzed for their ability to transfer genes to F recipient bacteria. The data shown below list the origin of transfer (oriT) for each strain and give the order of genes, with the first gene on the left and the last gene on the right. Hfr Strain Genes Transferred Hfr 1 Hfr 2 oriT met pro leu gal oriT ala lac thr azi oriT azi trp met pro Hfr 3 Use the...
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