Table 3: Absorbance for varying concentrations of Co(NO3)2 at max wavelength Sample Number Concentration (mM) Absorbance...
-
Table 3: Absorbance for varying concentrations of Co(NO3)2 at max wavelength
Answer the following questions based on your calibration curveSample Number
Concentration (mM)
Absorbance
0
0 mM
0.00
1
30 mM
0.13
2
60 mM
0.27
3
90mM
0.40
4
120mM
0.53
5
150 mM
0.67
- Calculate the
l value from the slope of your graph
- Calculate the
l value from the slope of your graphHomework Answers
| Sample | Concentration (in mM) | Absorbance |
| 0 | 0 | 0 |
| 1 | 30 | 0.13 |
| 2 | 60 | 0.27 |
| 3 | 90 | 0.4 |
| 4 | 120 | 0.53 |
| 5 | 150 | 0.67 |
From the graph, equation is
y=0.0045x - 0.001
Comparing with beer's law,
we get,
Add Answer to:
Table 3: Absorbance for varying
concentrations of Co(NO3)2 at max
wavelength
Sample Number
Concentration (mM)
Absorbance...
Table 5. Drink mix concentrations and absorbances for constructing a standard curve Concentration Concentration Absorbance (mm)...
Table 5. Drink mix concentrations and absorbances for constructing a standard curve Concentration Concentration Absorbance (mm) (M) Sample 1 0.51 Sample 2 Sample 3 Sample 4 How do I calculate to find (M)?
the guiding questions. the max absorbance is 460. Guiding questions complex turque . Compare the molar...
the
guiding questions. the max absorbance is 460.
Guiding questions complex turque . Compare the molar absorptivity coefficients you obtained with SCN and Fe" as limiting reagents. Would you expect them to be the same? Why or why not? (Week 1) • The accepted molar absorptivity coefficient for FeSCN is 7.0x10' M'cm''. Find the bias and relative bias of your values and suggest possible sources of errors. (Week 1) • Which molar absorptivity coefficient will you use to calculate the...
Enzyme Kinetics Lab Guide Table 2: Absorbance values per minute for varied substrate concentrations Time (min)...
Enzyme Kinetics Lab Guide Table 2: Absorbance values per minute for varied substrate concentrations Time (min) Tube A (0mM) Tube B (2.0mM) Tube C (5.0mM) Tube D (8.0mM) Tube E (12.0mM) Tube F (20.0mM) 0 0 0.012 0.066 0.066 0.076 0.191 1 0.011 0.045 0.128 0.128 0.164 0.200 2 0.017 0.096 0.190 0.190 0.219 0.241 3 0.009 0.122 0.234 0.234 0.294 0.313 Table 3. Absorbance values versus substrate concentration at a constant time (from Table 2) Concentration (mM) 0 mM...
To construct a calibration curve we would need to take our stock solution and dilute it...
To construct a calibration curve we would need to take our stock solution and dilute it to different concentrations. We would then test those different concentration in our spectrophotometer to test their absorbance. If we diluted our samples as follows, calculate the resulting concentrations. (HINT: this is dilution which means we can use M1V1=M2V2, where M1 is our original 150 mM concentration, V1 is the volume of the original concentration that we added water to dilute, M2 is the concentration...
1. Determining the Wavelength of Maximum Absorption: 2. Determining the concentration of an Unknown Solution Volume...
1. Determining the Wavelength of Maximum Absorption: 2. Determining the concentration of an Unknown Solution Volume of Stock Concentration of Soln #1 T2.001.xlon #2 5.00 2.5x loin . . x ^ 10.00 Soxom A=493.6 mm Rad y o sxlom Recorded Absorbance 0.212 0.528 0.947 1453 #4 Calibration Curve Slope of Least Squares Best Fit Line (molar abspoptivity, E) Equation for Least squares best fit line: A-42. Ubxk 2916xlor 6-01482 0.9652 Correlation Factor (R) for Least Squares Best Fit Line 3....
Operating Wavelength : 458.7 nm Preparation of Standard Calibration Curve of [FeSCN]2+ Sample Number Absorbance Final...
Operating Wavelength : 458.7 nm Preparation of Standard Calibration Curve of [FeSCN]2+ Sample Number Absorbance Final Concentration of [FeSCN]2+(M) A 0.157 4.0E-5 B 0.272 8.0E-5 C 0.422 1.2E-4 D 0.615 1.6E-4 E 0.722 2.0E-4 Determination of [FeSCN]2+ in Equilibrium Mixtures Sample Number Absorbance 1 0.240 2 0.334 3 0.436 4 0.544 Determination of [FeSCN]2+ in equilibrium mixtures For Sample #1: Enter the initial concentration of Fe3+ Calculate the equilibrium concentration of Fe3+ Enter the initial concentration of HSCN Calculate the...
Soln. 2.5x10-3 M Fe(NO3)3 mL 2.5X10-3 M KSCN mL 0.5 M HNO3 mL Absorbance A 5...
Soln. 2.5x10-3 M Fe(NO3)3 mL 2.5X10-3 M KSCN mL 0.5 M HNO3 mL Absorbance A 5 1 4 0.020 B 5 2 3 0.034 C 5 3 2 0.037 D 5 4 1 0.056 E 5 5 0 0.066 Calibrated at max wavelength of FeSCN2+ = 485.80nm Final concentration of Fe3+ = 5.56*10-5 Using M1V1=M2V2, calculate the initial concentrations of the reactant Fe3+, (m) and SCN- (n) in each solution A-E.
please help with question 1 and 3 part c Name: 8 9 Lab Section: 7 Pre-lab: The Concentration of Riboflavin 1. The gi...
please help with question 1 and 3 part c
Name: 8 9 Lab Section: 7 Pre-lab: The Concentration of Riboflavin 1. The given absorbance spectrum is recorded for a dye. Which is the most likely color of this dye? a. Blue-green b. Colorless c. Red d. Black M stock solution is diluted to 10.00 ml, what is the final 2. IF 4.00 ml of a 5.00 X 10 concentration? 3. The absorbance of known concentrations of blue food dye were...
Table 1. Concentrations of [Fe(Phen),1?, M, in water and UV- vis absorbance values recorded at 2...
Table 1. Concentrations of [Fe(Phen),1?, M, in water and UV- vis absorbance values recorded at 2 -505 nm. Concentration of [Fe(Phen)/, M Absorbance 9.500 x 10 0.999 4.250 x 10 0.480 0.219 2.125 10% 1.063 x 10 0.104 0.000 0.003 Table 2. UV-vis absorbance values of [Fe(phen)]* held at 35 °C after mixing with H SO, and recorded at 5 minute intervals (in water at max = 505 nm). Time (minutes) Absorbance 0.997 0.935 0.877 0.823 0.772 0.724 0.680 0.638...
please help answer questions for mixture 1 AND mixture 2 thank you! Table 23.1 Test Tube...
please help answer questions for mixture 1 AND mixture 2 thank
you!
Table 23.1 Test Tube Number 1 2 3 4 5 5,00 1.00 5.00 2.00 Volume Fe(NO), solution (ml.) Volume KSCN solution (ml.) Volume H,O (ml.) 5.00 3.00 2.00 5.00 4.00 5.00 5.00 0.00 4.00 3.00 1.00 Mixture 2 3 4 5 Absorbance 0.079 0.165 0.246 0.311 0.400 In order to convert the absorbance values provided below into equilibrium concentrations of FeSCN?, you will need to know the slope...
Table 5. Drink mix concentrations and absorbances for constructing a standard curve Concentration Concentration Absorbance (mm) (M) Sample 1 0.51 Sample 2 Sample 3 Sample 4 How do I calculate to find (M)?
the
guiding questions. the max absorbance is 460.
Guiding questions complex turque . Compare the molar absorptivity coefficients you obtained with SCN and Fe" as limiting reagents. Would you expect them to be the same? Why or why not? (Week 1) • The accepted molar absorptivity coefficient for FeSCN is 7.0x10' M'cm''. Find the bias and relative bias of your values and suggest possible sources of errors. (Week 1) • Which molar absorptivity coefficient will you use to calculate the...
1. Determining the Wavelength of Maximum Absorption: 2. Determining the concentration of an Unknown Solution Volume of Stock Concentration of Soln #1 T2.001.xlon #2 5.00 2.5x loin . . x ^ 10.00 Soxom A=493.6 mm Rad y o sxlom Recorded Absorbance 0.212 0.528 0.947 1453 #4 Calibration Curve Slope of Least Squares Best Fit Line (molar abspoptivity, E) Equation for Least squares best fit line: A-42. Ubxk 2916xlor 6-01482 0.9652 Correlation Factor (R) for Least Squares Best Fit Line 3....
please help with question 1 and 3 part c
Name: 8 9 Lab Section: 7 Pre-lab: The Concentration of Riboflavin 1. The given absorbance spectrum is recorded for a dye. Which is the most likely color of this dye? a. Blue-green b. Colorless c. Red d. Black M stock solution is diluted to 10.00 ml, what is the final 2. IF 4.00 ml of a 5.00 X 10 concentration? 3. The absorbance of known concentrations of blue food dye were...
Table 1. Concentrations of [Fe(Phen),1?, M, in water and UV- vis absorbance values recorded at 2 -505 nm. Concentration of [Fe(Phen)/, M Absorbance 9.500 x 10 0.999 4.250 x 10 0.480 0.219 2.125 10% 1.063 x 10 0.104 0.000 0.003 Table 2. UV-vis absorbance values of [Fe(phen)]* held at 35 °C after mixing with H SO, and recorded at 5 minute intervals (in water at max = 505 nm). Time (minutes) Absorbance 0.997 0.935 0.877 0.823 0.772 0.724 0.680 0.638...
please help answer questions for mixture 1 AND mixture 2 thank
you!
Table 23.1 Test Tube Number 1 2 3 4 5 5,00 1.00 5.00 2.00 Volume Fe(NO), solution (ml.) Volume KSCN solution (ml.) Volume H,O (ml.) 5.00 3.00 2.00 5.00 4.00 5.00 5.00 0.00 4.00 3.00 1.00 Mixture 2 3 4 5 Absorbance 0.079 0.165 0.246 0.311 0.400 In order to convert the absorbance values provided below into equilibrium concentrations of FeSCN?, you will need to know the slope...
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