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8. In the preparatory phase, which steps of reactions need(s) ATP as a substrate? 9. Lists all reactions including substrates

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(a) In the first step of the glycolytic pathway glucose is phosphorylated to glucose 6 phosphate at the expense on one ATP.

Glucose + ATP ----Glucose 6-phosphate +ADP + H±

(b)Reactions in the prepratory phase of glycolysis are as follows----

(1) Splitting Fructose 1,6-bisphosphate

Fructose 1,6-bisphosphate ---- Dihydroxyacetone phosphate + Glyceraldehyde 3-phosphate

(2)Triose Phosphate Isomerase.
Dihydroxyacetone phosphate (DHAP) <--- > Glyceraldehyde 3-phosphate

(3)Glyceraldehyde Phosphate Dehydrogenase. (Example of a reaction coupled to
oxidation/reduction)
Glyceraldehyde 3-phosphate (GAP) + Pi + NAD+ <=> 1,3-Bisphosphoglycerate (BPG) + NADH

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