the Nanodrop reading is 22.2
ng/ul.
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the Nanodrop reading is 22.2
ng/ul.
Question 2. (3 pts.) ng mg) IML Use your Nanodrop...
Short Answer (2 pts) (partial credit allowed) 3. When making a genomic DNA library, it is...
Short Answer (2 pts) (partial credit allowed) 3. When making a genomic DNA library, it is common to start with 10 ug of genomic DNA. Your liver preparation was diluted (luL with 249 uL of water) and the absorbance of the diluted sample at 260 nm was 0.035. How much of the undiluted sample will you need to use to make the library? (Show math for partial credit)
concentration of the stock is 39.2mg 3. (6 pts) Figure out your plan for making each...
concentration of the stock is 39.2mg
3. (6 pts) Figure out your plan for making each solution required in the protocol. Be certain you FINISH with the required volumes of EACH solution! a. Calculate what is needed to make the 4 mg/ml solution b. Calculate what is needed to make the 400 ug/ml working solution c. Calculate what is needed to make the 40 ug/ml working solution You are provided with the following portion of a protocol: Determine concentration of...
One method of measuring DNA is to use a spectrophotometer. A DNA solution absorbs light of...
One method of measuring DNA is to use a spectrophotometer. A DNA solution absorbs light of 260nm in proportion to concentration so that an optical density OD260 of 1 is equivalent to 50µg of DNA in 1mL of H2O. A student places 3µL of a DNA solution into a cuvette containing 997µL of H2O and obtains an OD260 of 0.034. What is the concentration of the original DNA sample in mg/mL? Please provide your answer to three decimal places and...
Question 14 4 pts A protein assay was performed using the Bradford Reagent to determine the...
Question 14 4 pts A protein assay was performed using the Bradford Reagent to determine the protein concentrations of a series of pet food extract samples. The BSA Standard Curve had a slope of 0.0173 Data obtained for the pet food are shown in the table below. Tubes 1-2 contained different volumes of the pet food extract. Tube # Volume Sample (UL) A(595) Volume Volume Water Reagent (3.0 (L) mL) 803 503 1 2 20 50 0.293 0.657 What is...
need help ASAP, all infromation is posted for each question Preparation #1: Azithromycin 500 mg IV...
need help ASAP, all infromation is posted for each question
Preparation #1: Azithromycin 500 mg IV Infusion Preparation: Transfer 500-mg of azithromycin solution to an appropriate volume of D5W. Case: You receive an order for Azithromycin 500-mg IV infusion (2-mg/mL final concentration) for the treatment of Community Acquired Pneumonia (CAP). The recommended infusion time at this concentration is 3 hours. Formula: per package insert Azithromycin 500-mg Dry Powder for Injection Vial 500-mg Sterile Water for Injection (SWFI) 4.8mL* D5W IV...
Your lab instructor has given you a protocol to perform a molecular cloning experiment. In a...
Your lab instructor has given you a protocol to perform a molecular cloning experiment. In a previous experiment, you used polymerase chain reaction (PCR) to amplify a sequence that you believe to regulate expression of a gene you are studying. You will now take this purified PCR product (double stranded DNA) and ligate it into a plasmid that contains a luciferase reporter gene. If your DNA sequence is a promoter sequence, then its presence will allow for expression of the...
explain question 6 please explain question 8 not 6 Mix 9 mL of water + 1...
explain question 6
please explain question 8 not 6
Mix 9 mL of water + 1 uL of DNA Mix 1 mL of water+9 mL of DNA QUESTION8 You make a 1:50 dilution of yoqr stock, then you make a 1:10 dilution of the 1:50. What is the dilution factor of the final solution? 1:100 ○ 1:500 1:10 1:5 QUESTION9 0.1 mL Click Save and Submit to save and submit.Click Save All Answers to save all answers Save All Answers...
Reading Question (Refer to Introduction section before answering the question): A student performs a coffee cup...
Reading Question (Refer to Introduction section before answering the question): A student performs a coffee cup calorimetry lab similar to the one you will be performing, An acid solution is mixed with a basic solution and the temperature change is recorded. The student calculates the following: quolution(surroundings) = 4011) What is the value of race ? Using the same procedure that will be performed to determine the enthalpy of tion, determine the enthalpy of reaction when magnesium metal is added...
How do you find the nominal KHP concentration and the empirical KHP concentration? What is the...
How do you find the nominal KHP concentration and the empirical
KHP concentration? What is the difference between the two?
VOLUMETRIC ANALYSIS REVIEW Reference Data NaoH Concentration: 00314m KHIP Fomu HC&HC4 xI Mlr Mas 20.23g/ml Balanced Equation for Reaction :EHC8H40ч t lUaHiluahC8Htdar) t Mass of KHP used:00 KHP Molar Mass Preparation Data H2OC) 1001お - Moles of KHP: show work below y.13xld'3noi volume ofKHP Solution:-100mL no\ Nominal KHP Concentration :. show work below 0.0043 Titration Data Trial 1 Trial 2...
Question 5 1 pts Keep in mind that the absorbance was plotted on the y-axis and...
Question 5 1 pts Keep in mind that the absorbance was plotted on the y-axis and concentration of Fe in mg/mL was plotted on x-axis to get best-fit line equation from the standard solutions. If the best fit line equation is y = 100x +0.01 What is the Fe concentration in mg/mL if the absorbance is 0.4 based on the best-fit line equation? Keep two sig figs for your answer. The Total Cereal has a Fe concentration of 0.6 g...
Short Answer (2 pts) (partial credit allowed) 3. When making a genomic DNA library, it is common to start with 10 ug of genomic DNA. Your liver preparation was diluted (luL with 249 uL of water) and the absorbance of the diluted sample at 260 nm was 0.035. How much of the undiluted sample will you need to use to make the library? (Show math for partial credit)
concentration of the stock is 39.2mg
3. (6 pts) Figure out your plan for making each solution required in the protocol. Be certain you FINISH with the required volumes of EACH solution! a. Calculate what is needed to make the 4 mg/ml solution b. Calculate what is needed to make the 400 ug/ml working solution c. Calculate what is needed to make the 40 ug/ml working solution You are provided with the following portion of a protocol: Determine concentration of...
Question 14 4 pts A protein assay was performed using the Bradford Reagent to determine the protein concentrations of a series of pet food extract samples. The BSA Standard Curve had a slope of 0.0173 Data obtained for the pet food are shown in the table below. Tubes 1-2 contained different volumes of the pet food extract. Tube # Volume Sample (UL) A(595) Volume Volume Water Reagent (3.0 (L) mL) 803 503 1 2 20 50 0.293 0.657 What is...
need help ASAP, all infromation is posted for each question
Preparation #1: Azithromycin 500 mg IV Infusion Preparation: Transfer 500-mg of azithromycin solution to an appropriate volume of D5W. Case: You receive an order for Azithromycin 500-mg IV infusion (2-mg/mL final concentration) for the treatment of Community Acquired Pneumonia (CAP). The recommended infusion time at this concentration is 3 hours. Formula: per package insert Azithromycin 500-mg Dry Powder for Injection Vial 500-mg Sterile Water for Injection (SWFI) 4.8mL* D5W IV...
explain question 6
please explain question 8 not 6
Mix 9 mL of water + 1 uL of DNA Mix 1 mL of water+9 mL of DNA QUESTION8 You make a 1:50 dilution of yoqr stock, then you make a 1:10 dilution of the 1:50. What is the dilution factor of the final solution? 1:100 ○ 1:500 1:10 1:5 QUESTION9 0.1 mL Click Save and Submit to save and submit.Click Save All Answers to save all answers Save All Answers...
Reading Question (Refer to Introduction section before answering the question): A student performs a coffee cup calorimetry lab similar to the one you will be performing, An acid solution is mixed with a basic solution and the temperature change is recorded. The student calculates the following: quolution(surroundings) = 4011) What is the value of race ? Using the same procedure that will be performed to determine the enthalpy of tion, determine the enthalpy of reaction when magnesium metal is added...
How do you find the nominal KHP concentration and the empirical
KHP concentration? What is the difference between the two?
VOLUMETRIC ANALYSIS REVIEW Reference Data NaoH Concentration: 00314m KHIP Fomu HC&HC4 xI Mlr Mas 20.23g/ml Balanced Equation for Reaction :EHC8H40ч t lUaHiluahC8Htdar) t Mass of KHP used:00 KHP Molar Mass Preparation Data H2OC) 1001お - Moles of KHP: show work below y.13xld'3noi volume ofKHP Solution:-100mL no\ Nominal KHP Concentration :. show work below 0.0043 Titration Data Trial 1 Trial 2...
Question 5 1 pts Keep in mind that the absorbance was plotted on the y-axis and concentration of Fe in mg/mL was plotted on x-axis to get best-fit line equation from the standard solutions. If the best fit line equation is y = 100x +0.01 What is the Fe concentration in mg/mL if the absorbance is 0.4 based on the best-fit line equation? Keep two sig figs for your answer. The Total Cereal has a Fe concentration of 0.6 g...
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