Question

Hi,

please make the graph below is given the data. and also, comment on the following data given. please so it asap. thanks

Tule 1 Standard Curve Tube # Abs. mg/ml PNP Use your standard curve to calculate molar absorptivity coefficient of PNP? Show work. 0.133 0.264 0:530 0.95 0 0.063 0.125 0.25 0.5

Answer 1

The plots are provided first, then discussion is provided.

y = 2.088X-0.012 R?=0.996 0.2 0.4 0.6 0.8 1 1.2

0.87 0.7 0.6 y= 0.005x-0.125 R=0.992 0.5 Absorbance 0.4 0.3 0.2 0.1 0 20 40 60 120 140 160 80 100 time (sec)

Abs vs Substrate Concentration y= 0.004x -0.062 R = 0.958 y = 0.112e0.010% R? = 0.981 0 50 100 150 200 250

Abs vs Substrate Concentration y=0.004x -0.062 R = 0.958 y=0.112e 0.010x R = 0.981 50 100 150 200 250

plot of Abs vs In(T) y=0.490ln(x) - 1.550 R? = 0.989 0 10 20 30 40 50 60 70 80

1 from the straight line; slope = 2,188 mg mi Thus the molar absorbity efficient of PNP = 2.088 mg alme :A: ECL) 2 with time, the enzymatic reaction to progresses linearly with increasing substrate concentration, reaction rate increase exponentially one could also give a linear fit, but better fit as evidenced by R² shows exponential nature to be more accurate than linear a) from plot of Absorbance vs pH 10 appears liner. 5) characteristic of Arrhenius reactions, the blot looks exponential.