Question

1. What does each disc have a precise amount of? SLIDE 3 KIRBY-BAUER ANTIBIOTIC SENSITIVITY PLATE USING MEULLER-HINTON AGAR 2. Does a large zone of inhibition automatically qualify the antibiotic as effective and thus the organisms (sensitive)? 3. How do you know if any one of these zones of inhibition deems the organisms (sensitive) to the antibiotic? 4. What does this agar (MEULLER-HINTON AGAR) control for (how does this agar put all the antibiotics on an equal footing)? 5. Circle any antibiotics that are likely R (resistant) 6. What do we call an antibiotic that an organism is sensitive to, but does not achieve a zone of inhibition large enough in ?

Answer 1

1. Each disc contain a precise amount of antibiotic.

2. Apparently yes ,beacuse a large zone of inhibition shows a significant effect of antibiotic on bacterial growth by producing a zone of inhibition.

3. Large zone of inhibition seems the organisms sensitive to antibiotic.

4. Meuller-hinton agar has loose agar that allow better diffusion of antibiotics and help in equal distribution of antibiotic and it contains starch as well that can absorb toxic substances released by bacteria that may interfere with antibiotics .

5. That antibiotic is bacteriostatic i.e inhibiting the growth of bacteria and not killing the bacteria.