Question

An individual presents with a positive antibody screen. The antibody reacts with one antibody screen cell in the AHG phase.

1. What antibodies are possible based on the reactivity phase?
2. What temperature is necessary for reactivity in this phase?
3. This patient needs a transfusion. What special steps should be taken to assure this patient receives compatible blood?

Answer 1

Answer :

Antibody screening is done in 3 phases

A) Immediate spin phase

B) 37°C phase

C) AHG phase

Here one antibody screening is positive in AHG phase

1) AHG detects bound RBC antibodies that do not produce direct agglutination (sensitising antibodies).They are usually clinically significant IgG type of antibody .

2) AHG phase reaction occurs at room temperature (18°-25°C).

3) If screening result is positive for any phase, more tests should be done to recognise the specificity of the antibody. Patient should be provided antigen negative blood as dictated by test reports .

Answer 2

A "minicold panel" was tested on blood and yielded the following results: SC I SCII Cord 1 Cord 2 AUTO4degreesC 3+ 3+ 0 0 3+ 1) What is the probable specificity of the antibody?2) What procedures would avoid the antibodies serologic reactivity?