Question

Use the survey information to help form a hypothesis of whether you are phenotypically a “taster” or “non-taster”.

Look at your scores for orange juice, carrots, eggplant, green beans, sprouts, and potatoes crossing them off as you go down the list. – Do you dislike most of these? Perhaps you are a picky eater.

The rest of the foods have been placed on the list based on reports that some tasters find the food bitter and/or due to the known presence of thiourea containing substances within these foods (Drewnowski et al., 2001; Laaksonen et al., 2013; Sandell & Breslin, 2006; Wooding, 2006).

ܩ (1 3 3 3 3 3 3 4 4 4 4 4 4 Grapefruit juice Orange Juice Cabbage Spinach (large raw) 1 Carrot 1 Coffee (black) 1 Turnip Eggplant 1 Brussel sprouts 1 Raw Broccoli 1 Green beans 1 Tofu 1 Potato 1 Red Radish 1 Kale 1 Sprouts (alfalfa) 1 5 5 N/A 5 N/A 5 N/A 5 NA 5 NA 5 N/A w w w w w w w w w w w w NNNNNNNNNN ܩ ܩ 3 3 3 3 3 3 3 3 4 4 4 4 4 4 4 4 5 N/A 5 N/A 5 N/A 5 NA 5 N/A 5 N/A 5 N/A 5 NA ܩ ܩ ܩ ܩ What is your hypothesis? I am phenotypically a non-taster. If your hypothesis is correct what is/are your possible genotype/s? Phenotype - PTC Taste Test Prediction: Based on your hypothesis above would the PTC strip taste bitter or like wet paper? Wet paper Your predicted phenotype

Answer 1

Predicted Phenotype- Non- Tester.

Will Your PCR product be Digested with HaeIII enzyme ? - No. Bacuse this Restriction site is only prasent in the tester allele of the TAS2R38 gene.

Based on the Hypothesis above do you expect a band around 203bp for your digested Sample- Yes. If i am a true Non-tester i will have a band at 203bp.

Do You expect a band on 156bp- No. bacsue me being a non tester, my DNA will lack the restriction site at TAS2R38 locus. so there will be no cleavage by HaeIII enzyme and there will be no band at 156 bp (while for True testers and moderate testers there will be a HaeIII cut site resulting in the presence of 156bp band in the DNA electrophoreisis gel).

What is your genotype- tt.

Based On the Above hyothesis i am a - Homozygous Non Tester (tt)

Possible genotypes of the perents - either Both Homozygous non-testers (tt) or Both heterozygous(Tt) or one Heterozygous (Tt) One homozygous non tester (tt).

Punnet Square

Female

T t

T TT(Tester) Tt (Heterozygous)

Male

t Tt (Heterozygous) tt (Non Tester)

No, Based on My predicted genotype, my children can never be testers as,

Case 1- If my wife is tt (non tester)

all my children will be non testers as there is no gene in both parents fo tester phenotype T.

Case 2- If my wife is a heterozygous (Tt)

Half of the children will be heterozygous (Tt) and half non testers (tt)

Case 3- If my wife is a tester (TT)

All the children will be heterozygous (Tt).