To successfully express a protein from a eukaryotic expression vector, the following is NOT required. a)...
To successfully express a protein from a eukaryotic expression vector, the following is
NOT required.
a) Selectable marker
b) Ribosome binding site
c) Strong eukaryotic promoter
d) Termination sequence
Homework Answers
Selectable marker is not required to successfully express a protein from a eukaryotic expression vector.
Add Answer to:
To successfully express a protein from a eukaryotic expression
vector, the following is
NOT required.
a)...
The abundance of mRNA in mammalian cells is measured to be: a)1% of total RNA content...
The abundance of mRNA in mammalian cells is measured to be: a)1% of total RNA content b)10% of total cell content c)80% of total RNA content d)The same as protein content 2. RNAi is used for one of the following purposes. a) To disrupt a DNA b) To clone a particular gene c) To verify the function of a gene d) To produce a new gene To successfully express a protein from a eukaryotic expression vector, the following is NOT...
1. The ribosome is important for catalyzing the formation of peptide bonds. Which of the following...
1. The ribosome is important for catalyzing the formation of peptide bonds. Which of the following statements is true? (a) The number of rRNA molecules that make up a ribosome greatly exceeds the number of protein molecules found in the ribosome. (b) The large subunit of the ribosome is important for binding to the mRNA. (c) The catalytic site for peptide bond formation is formed primarily from an rRNA. (d) Once the large and small subunits of the ribosome assemble,...
Outline the steps required to close a eukaryotic gene using E. coli from mRNA derived from...
Outline the steps required to close a eukaryotic gene using E. coli from mRNA derived from cells expressing the gene. Include how you would amplify the specific gene and how you would select for bacterial cells containing the cloned gene if the gene was inserted into a multiple cloning site within the lacZ gene on a plasmic. If you wanted to later express the gene using a eukaryotic expression vector and promoter how would you ensure the gene was in...
Your friend decides to place Green Fluorescent Protein (GFP) under the control of the promoter from...
Your friend decides to place Green Fluorescent Protein (GFP)
under the control of the promoter from the lacZ gene we discussed
in class. She put this expression plasmid into a bacterium. The
promoter from the lacZ gene is diagrammed to the right.?
2) Your friend decides to place Green Fluorescent Protein (GFP) under the control of the promoter from the lacZ gene we discussed in class. She put this expression plasmid into a bacterium. The promoter from the lacZ gene...
3. Prokaryotic and eukaryotic gene expression compared. Below is an incomplete table of prokaryotic and eukaryotic...
3. Prokaryotic and eukaryotic gene expression compared. Below is an incomplete table of prokaryotic and eukaryotic gene expression in comparison. Fill in the blank using PPT slides, notes and the textbook. Prokaryotic gene expression Eukaryotic gene expression Overview Steps Transcription and translation Yes Transcription and translation coupled? Gene structure No introns Epigenetic modification (chromosome remodeling) transcription, translation, RNA processing, protein processing Transcription in the nucleus and translation in the cytoplasm Interrupted gene with exons and introns RNAPI, II, III Which...
Which of the following correctly describes eukaryotic transcriptional control? a. A transcription factor is a...
Which of the following correctly describes eukaryotic transcriptional control? a. A transcription factor is a DNA molecule that helps RNA polymerase to bind to the enhancer of a specific gene. b. An enhancer is a protein that encourages gene expression by binding to the DNA. c. The promoter is the region of RNA where DNA polymerase will bind to begin transcription. d. The interaction of multiple transcription factors may be required in order to transcribe a...
can you guys please give me the correct answers and explain why? 19. You clone your...
can you guys please give me the correct answers and explain
why?
19. You clone your favorite E. coli gene including the promoter region, the open reading frame, and some flanking DNA. You determine the DNA sequence of the entire region and map the start site of transcription. You note the following sequence near the end o your gene. What is the likely function of this sequence? ...CCCAGCCCGCCTAATGAGCGGGCTTTTTTTTTTGAAGGTATAT... A. A polyadenylation signal B. A Rut site C. The ribosome binding...
21. Double helix 22. Repressor protein 23. Adenine 24. Ribosome.
21. Double helix22. Repressor protein23. Adenine24. Ribosome.25. Promoter26. Replication27. RNA Polymerase.28. CodonA. Enzyme that synthesizes RNAB. Organelle where proteins are assembledC. Complementary to either Thymine or UracilD. mRNA sequence that codes for one amino acidE. Shape of double stranded DNAF. Sequence of DNA that controls gene expressionG. binds an operator and stops gene expression in LAC operon by preventing RNA polymerase from binding gene and transcribing. H. Duplication of DNA in 5 phase of Interphase
Below is the partial coding strand of DNA for a gene containing an ORF, shown 5'...
Below is the partial coding strand of DNA for a gene containing
an ORF, shown 5' to 3'. Identify the regions, shown 1 to 9,
associated with the following genetic elements. Some elements may
have more than one associated region.
(a) The promoter region
(b) The ribosome binding site
(c) The ribosome binding site consensus
sequence
(d) The start codon
(f) The expected region for the +1
transcription (where the transcript
begins to be made)
(g) Is this a prokaryotic...
Immunology Help 1) Expression of functional beta chain protein signals that a thymocyte has successfully reached...
Immunology Help 1) Expression of functional beta chain protein signals that a thymocyte has successfully reached what stage of development? A The pro-T cell stage B The pre-T cell stage C The double-negative stage D The double-positive stage E The single-positive stage 2) Which one of the following types of antibodies is least likely to be found in normal lymph fluid? A IgA B IgD C IgE D IgG E IgM 3) Which CDR is found closest to the center...
Your friend decides to place Green Fluorescent Protein (GFP)
under the control of the promoter from the lacZ gene we discussed
in class. She put this expression plasmid into a bacterium. The
promoter from the lacZ gene is diagrammed to the right.?
2) Your friend decides to place Green Fluorescent Protein (GFP) under the control of the promoter from the lacZ gene we discussed in class. She put this expression plasmid into a bacterium. The promoter from the lacZ gene...
3. Prokaryotic and eukaryotic gene expression compared. Below is an incomplete table of prokaryotic and eukaryotic gene expression in comparison. Fill in the blank using PPT slides, notes and the textbook. Prokaryotic gene expression Eukaryotic gene expression Overview Steps Transcription and translation Yes Transcription and translation coupled? Gene structure No introns Epigenetic modification (chromosome remodeling) transcription, translation, RNA processing, protein processing Transcription in the nucleus and translation in the cytoplasm Interrupted gene with exons and introns RNAPI, II, III Which...
can you guys please give me the correct answers and explain
why?
19. You clone your favorite E. coli gene including the promoter region, the open reading frame, and some flanking DNA. You determine the DNA sequence of the entire region and map the start site of transcription. You note the following sequence near the end o your gene. What is the likely function of this sequence? ...CCCAGCCCGCCTAATGAGCGGGCTTTTTTTTTTGAAGGTATAT... A. A polyadenylation signal B. A Rut site C. The ribosome binding...
Below is the partial coding strand of DNA for a gene containing
an ORF, shown 5' to 3'. Identify the regions, shown 1 to 9,
associated with the following genetic elements. Some elements may
have more than one associated region.
(a) The promoter region
(b) The ribosome binding site
(c) The ribosome binding site consensus
sequence
(d) The start codon
(f) The expected region for the +1
transcription (where the transcript
begins to be made)
(g) Is this a prokaryotic...
Most questions answered within 3 hours.
-
Calculate the pH of each of the following solutions.
0.50 M HBr
3.1×10−4 M KOH
4.2×10−5...
asked 2 hours ago -
For the year ended December 31, Depot Max’s cost of merchandise
sold was $85,600. Inventory at the...
asked 2 hours ago -
Week 10 - Professional Memo Assignment
Professional Memo Assignment
Your mission for this week, should you...
asked 2 hours ago -
Write a Python program that stores the data for each
player on the team, and it...
asked 2 hours ago -
In
the last 3 months, mike never knows when he is going to get his
allowance...
asked 2 hours ago -
Is Ca(OH)2 a Bronsted base, Lewis base, or both? Why?
asked 2 hours ago -
1A- Why don’t voters complain about U.S. tariffs on imported
sugar?
Because sugar is only a...
asked 2 hours ago -
Cash Payback Period
Primera Banco is evaluating two capital investment proposals for
a drive-up ATM kiosk,...
asked 2 hours ago -
Create a button in Swift (Xcode) that will create a charge,
create a charge using Stripe's...
asked 2 hours ago -
The reaction rate of CO and NO2 in the reaction
CO(g) + NO2(g) → CO2(g) +...
asked 2 hours ago -
Imagine that a chemist puts 6.40 mol each of
C3H8 and O2 in a 1.00-L container...
asked 2 hours ago -
How much money should be invested today in order to have $8340
at the end of...
asked 2 hours ago