Homework Answers
Option A - Rec A
Rec A protein binds to the single stranded dna during homologous recombination event. The ssDNa-Rec A complex then evades the double stranded DNA and look for sequence similarity.It includes stretching of the ds DNA to enhance sequence complementarity identification.Rec A protein makes the complete recomnrecombi possible by creating a heteroduplex thousands of base pairs long.
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Which protein binds to single-stranded DNA, allowing the single-stranded DNA to invade a double-stranded DNA double...
Viruses are composed of O proteins, RNA and ribosomes O single stranded DNA O double stranded...
Viruses are composed of O proteins, RNA and ribosomes O single stranded DNA O double stranded DNA and RNA Single stranded RNA O RNA or DNA, but never both, and a protein coat
21. Double helix 22. Repressor protein 23. Adenine 24. Ribosome.
21. Double helix22. Repressor protein23. Adenine24. Ribosome.25. Promoter26. Replication27. RNA Polymerase.28. CodonA. Enzyme that synthesizes RNAB. Organelle where proteins are assembledC. Complementary to either Thymine or UracilD. mRNA sequence that codes for one amino acidE. Shape of double stranded DNAF. Sequence of DNA that controls gene expressionG. binds an operator and stops gene expression in LAC operon by preventing RNA polymerase from binding gene and transcribing. H. Duplication of DNA in 5 phase of Interphase
The activity of would be quickly undone in the absence of single-stranded binding protein. O DNA...
The activity of would be quickly undone in the absence of single-stranded binding protein. O DNA polymerase | O DNA polymerase III O DNA ligase O DNA helicase O primase
1. The figure below shows a DNA double helix and its nucleotide sequence. A. A homodimeric...
1. The figure below shows a DNA double helix and its nucleotide
sequence.
A. A homodimeric helix–turn–helix protein binds to a DNA
fragment containing this sequence. Its preferred half-site is
AACAC. Show where the two recognition helices in the protein
contact the DNA.
B. Are protein–DNA contacts primarily in the major or the minor
groove of DNA? What parts of the nucleotides contact the amino acid
side chains of the protein to provide most sequence specificity?
What kind of bond...
(2) In isolation, a DNA-binding protein binds to its regulatory sequence with a Kd of 1.0 M. Another DNA binding protein binds to another sequence on the same DNA a few bases away with a Kd of 5....
(2) In isolation, a DNA-binding protein binds to its regulatory sequence with a Kd of 1.0 M. Another DNA binding protein binds to another sequence on the same DNA a few bases away with a Kd of 5.0 HM when alone. The two proteins each have a domain which binds to the other with an interaction energy of -2.7 kcal/mole: (a) Draw the thermodynamic box which represents all four states of this system (b) what are the affinities for each...
3) DNA polymerases that have an editing function bind to and encircle double-stranded DNA in their...
3) DNA polymerases that have an editing function bind to and encircle double-stranded DNA in their "palm" domain, and grip the double-stranded DNA tightly between their "thumb" and "fingers" domains. Since they can bind to any DNA double helix regardless of its sequence, which groove in DNA do you think they sample ("feel") for correct helix geometry? a) The minor groove b) The major groove c) Either of the above is equally probable
DNA-binding proteins are proteins that are composed of DNA-binding domains and thus have a specific or...
DNA-binding proteins are proteins that are composed of DNA-binding domains and thus have a specific or general affinity for either single or double stranded DNA. Protein X is one such protein that binds to double stranded DNA. What residues are most likely to be located in the DNA binding site of protein X and why?
Consider the double-stranded DNA sequence of a gene below: Strand 1 5'- AATCGTATGCGAAGCCCTTAACT-3' Strand 2. С...
Consider the double-stranded DNA sequence of a gene below: Strand 1 5'- AATCGTATGCGAAGCCCTTAACT-3' Strand 2. С стената. 3. TTAGCATACGCTTCGGGAATTGA-5' The number of amino acids in the corresponding peptide is: OA.O B.1 OC3 0.4 O E.5 Consider the following double-stranded region of a gene: Strand 17 5'- AATCGTATGCGAAGCCCTTAACT-3 Strand 2A 3'-TTAG CATACGCTTCGGGAATTGA-5 The number of mRNA codons in the corresponding transcriptis O A1 OB.4 OC.5 OD.6 E. Cannot be determined
20. Which enzyme separates the strands of the DNA helix? A. DNA Polymerase E. Single Stranded...
20. Which enzyme separates the strands of the DNA helix? A. DNA Polymerase E. Single Stranded Binding Proteins B. Ligase F. Primase C. Helicase G. Lagging Strand D. Topoisomerase H. Leading Strand 21. Which enzyme joins newly synthesized DNA fragments on the lagging strand? A. DNA Polymerase E. Single Stranded Binding Proteins B. Ligase F. Primase C. Helicase G. Lagging Strand D. Topoisomerase H. Leading Strand 22. In a PCR reaction, at which temperature do the two strands of DNA...
Retroviruses such as HIV have o two copies of single-stranded DNA o one copy of single-stranded...
Retroviruses such as HIV have o two copies of single-stranded DNA o one copy of single-stranded RNA. o two single-stranded copies of capsid protein. o two copies of single-stranded RNA o one copy of double-stranded RNA.
Viruses are composed of O proteins, RNA and ribosomes O single stranded DNA O double stranded DNA and RNA Single stranded RNA O RNA or DNA, but never both, and a protein coat
The activity of would be quickly undone in the absence of single-stranded binding protein. O DNA polymerase | O DNA polymerase III O DNA ligase O DNA helicase O primase
1. The figure below shows a DNA double helix and its nucleotide
sequence.
A. A homodimeric helix–turn–helix protein binds to a DNA
fragment containing this sequence. Its preferred half-site is
AACAC. Show where the two recognition helices in the protein
contact the DNA.
B. Are protein–DNA contacts primarily in the major or the minor
groove of DNA? What parts of the nucleotides contact the amino acid
side chains of the protein to provide most sequence specificity?
What kind of bond...
(2) In isolation, a DNA-binding protein binds to its regulatory sequence with a Kd of 1.0 M. Another DNA binding protein binds to another sequence on the same DNA a few bases away with a Kd of 5.0 HM when alone. The two proteins each have a domain which binds to the other with an interaction energy of -2.7 kcal/mole: (a) Draw the thermodynamic box which represents all four states of this system (b) what are the affinities for each...
3) DNA polymerases that have an editing function bind to and encircle double-stranded DNA in their "palm" domain, and grip the double-stranded DNA tightly between their "thumb" and "fingers" domains. Since they can bind to any DNA double helix regardless of its sequence, which groove in DNA do you think they sample ("feel") for correct helix geometry? a) The minor groove b) The major groove c) Either of the above is equally probable
Consider the double-stranded DNA sequence of a gene below: Strand 1 5'- AATCGTATGCGAAGCCCTTAACT-3' Strand 2. С стената. 3. TTAGCATACGCTTCGGGAATTGA-5' The number of amino acids in the corresponding peptide is: OA.O B.1 OC3 0.4 O E.5 Consider the following double-stranded region of a gene: Strand 17 5'- AATCGTATGCGAAGCCCTTAACT-3 Strand 2A 3'-TTAG CATACGCTTCGGGAATTGA-5 The number of mRNA codons in the corresponding transcriptis O A1 OB.4 OC.5 OD.6 E. Cannot be determined
20. Which enzyme separates the strands of the DNA helix? A. DNA Polymerase E. Single Stranded Binding Proteins B. Ligase F. Primase C. Helicase G. Lagging Strand D. Topoisomerase H. Leading Strand 21. Which enzyme joins newly synthesized DNA fragments on the lagging strand? A. DNA Polymerase E. Single Stranded Binding Proteins B. Ligase F. Primase C. Helicase G. Lagging Strand D. Topoisomerase H. Leading Strand 22. In a PCR reaction, at which temperature do the two strands of DNA...
Retroviruses such as HIV have o two copies of single-stranded DNA o one copy of single-stranded RNA. o two single-stranded copies of capsid protein. o two copies of single-stranded RNA o one copy of double-stranded RNA.
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