Question

Problem 1. (15 pts) Multiple Choice Which of the following methods can be used to purify large amounts of proteins a. Hydrophobic chromatography b. Precipitation c. Equilibrium dialysis d. Polyacrylamide gel electrophoresis e. Ion exchange chromatography f. a, c, e g. a, b, c, d h. a, b, c, e i. All of the above Circle the one correct answer. a. Beta sheets are stabilized by H-bonds b. Ramachandran plots are determined by steric clashes between side chains and the carbonyl oxygen in the polypeptide backbone c. Proline is never found in alpha helices d. Parallel beta sheets are more stable than anti-parallel beta sheets e. a, b, c f. a, b, d g. a, c, d a. All of the above

Answer 1

1 Q (Ans): Methods that can be used to purify large amounts of protein are Hydrophobic chromatography, Precipitation, Equilibrium dialysis, and Iron exchange chromatography

Polyacrylamide gel electrophoresis is an analytical method of protien purification and that cannot be used to purify large amounts of protein

So the answer is option h

2 Q (Ans): a, b, c are correct.

Unparallel beta-sheets are more stable than parallel beta-sheets, so d is incorrect

So the answer is Option e