Which of the following would you choose to study the role of polymerase subunits in elongation?...
Which of the following would you choose to study the role of
polymerase subunits in elongation?
A. epitope tagging
B. immunoprecipitation
c. in vitro transcription
D. western blotting
E. DNAase footprinting
Homework Answers
Answer : c. In vitro transcription
Explanation : In vitro transcription we can synthesize as much as RNA we want for any application. During elongation of RNA transcription bacteriophage λ gene N and Q protein control anti termination of RNA synthesis. In vitro transcription use bacteriophage DNA-dependent RNA polymerase ( T7, T3, SP6) RNA polymerase to synthesize RNA from a DNA template. So in the time in vitro transcription we study the role of polymerase subunit in elongation.
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Which of the following would you choose to study the role of
polymerase subunits in elongation?...
QUESTION 19 Which of the following subunits plays no role in chain elongation during transcription? O...
QUESTION 19 Which of the following subunits plays no role in chain elongation during transcription? O a) RNA polymerase holoenzyme O b) sigma factor O c) RNA polymerase core enzyme O d) all of the above O e) none of the above
QUESTION Which of the following would you use to find a particular sequence within a sample...
QUESTION Which of the following would you use to find a particular sequence within a sample of DNA? O RT-PCR O Western blotting O Northern blotting O Southern blotting QUESTION 7 The Sanger method for DNA sequencing requires 。A. dideoxynucleotides O8. DNA nucleotides OC DNA polymerase O D.A&B O E. A, B, & C .
Which of the following is NOT a function of transcription that requires the activity from subunits...
Which of the following is NOT a function of transcription that requires the activity from subunits of the Core RNA Palymerase? a. RNA polymerase activity that base-pairs and polymerizes nucleotides to make mRNA. b. Helicase activity that unwinds the double-stranded DNA molecule for transcription c. Specific recognition of -35 box and -10 box sites in the promoter region. d. General binding that helps RNA polymerase loosely adhere to DNA, before Transcription begins. Oe. Trick Question. The Core RNA polymerase can...
Compared to RNA elongation by RNA polymerases, DNA elongation by DNA polymerase requires an additional reaction...
Compared to RNA elongation by RNA polymerases, DNA elongation by DNA polymerase requires an additional reaction component. Which is appropriate for fulfillment of this requirement? Select one: a. a primase is needed for the biological DNA replication. b. a short DNA primer is necessary for PCR. c. a host tRNA is used for the synthesis of DNA by viral reverse transcriptase. d. All of these e. None of these
1. Which of the following organisms would produce functional lac products in the presence of IPTG?...
1. Which of the following organisms would produce functional lac products in the presence of IPTG? A. Is Oc Z- Y+ A+ B. Is Oc Z- Y- A- C. I+ O+ Z+ Y+ A+ D. I-Oc Z+ Y+ A+ E. Both Is Oc Z- Y+ A+ and Is Oc Z- Y+ A+ are correct 2. Which of the following statements is true about a lac operon with this genotype? I-d O+ Z+ Y- A- A. The operon is repressible. B....
Choose all that apply to the initiation of transcription Promoter segments Ribosome tRNA DNA polymerase Transcription...
Choose all that apply to the initiation of transcription Promoter segments Ribosome tRNA DNA polymerase Transcription factors Question 25 (4 points) Saved Choose all the molecules that are used in transcription: a) primase b) DNA c) nucleotides d) RNA polymerase e) SSB (single strand binding protein)
Which of the following is NOT a difference between DNA and RNA synthesis? a. the nature...
Which of the following is NOT a difference between DNA and RNA synthesis? a. the nature of the nucleotide used b. the direction of synthesis c. the presence of discrete termination signals d. the simultaneous coding from both strands of DNA. During transcription, separation of the DNA strands is necessary. This involves: a. During transcription, separation of the DNA strands is necessary. This involves: b. separation of long segments of the DNA corresponding to genes. c. separation in only a...
Which of the following enzymes catalyzes the elongation of a nucleic strand in the 3'-->5' direction?...
Which of the following enzymes catalyzes the elongation of a nucleic strand in the 3'-->5' direction? A) DNA polymerase I ***This is the answer choice I originally put and it was incorrect*** B) DNA polymerase III C) RNA primase D) Reverse transcriptase E) All of the above
42. Which is correct about the peptide elongation by ribosome? Select one: a. Addition of a...
42. Which is correct about the peptide elongation by ribosome? Select one: a. Addition of a new amino acid is achieved by peptide transfer from the A site tRNA to the P site URNA. b. Translation is not affected by the cellular availability of GTP. O c. Peptide bond formation is catalyzed by enzymatic proteins in ribosome. O d. The peptide bond is formed by a nucleophilic attack of the A-site NH2- onto the P-site -COOH. O e. None of...
Which of the following is true for transcription in E. coli, but not for transcription in...
Which of the following is true for transcription in E. coli, but not for transcription in humans? a. Transcription requires RNA polymerase to add nucleoside triphosphates to the 3' end of the growing transcript (RNA). b. Transcription does not require primers during initiation or elongation. c. Transcription requires promoters, which have mostly adenines and thymines. d. Transcription produces polycistronic mRNA that includes several coding regions within one transcription unit.
QUESTION 19 Which of the following subunits plays no role in chain elongation during transcription? O a) RNA polymerase holoenzyme O b) sigma factor O c) RNA polymerase core enzyme O d) all of the above O e) none of the above
QUESTION Which of the following would you use to find a particular sequence within a sample of DNA? O RT-PCR O Western blotting O Northern blotting O Southern blotting QUESTION 7 The Sanger method for DNA sequencing requires 。A. dideoxynucleotides O8. DNA nucleotides OC DNA polymerase O D.A&B O E. A, B, & C .
Which of the following is NOT a function of transcription that requires the activity from subunits of the Core RNA Palymerase? a. RNA polymerase activity that base-pairs and polymerizes nucleotides to make mRNA. b. Helicase activity that unwinds the double-stranded DNA molecule for transcription c. Specific recognition of -35 box and -10 box sites in the promoter region. d. General binding that helps RNA polymerase loosely adhere to DNA, before Transcription begins. Oe. Trick Question. The Core RNA polymerase can...
Choose all that apply to the initiation of transcription Promoter segments Ribosome tRNA DNA polymerase Transcription factors Question 25 (4 points) Saved Choose all the molecules that are used in transcription: a) primase b) DNA c) nucleotides d) RNA polymerase e) SSB (single strand binding protein)
42. Which is correct about the peptide elongation by ribosome? Select one: a. Addition of a new amino acid is achieved by peptide transfer from the A site tRNA to the P site URNA. b. Translation is not affected by the cellular availability of GTP. O c. Peptide bond formation is catalyzed by enzymatic proteins in ribosome. O d. The peptide bond is formed by a nucleophilic attack of the A-site NH2- onto the P-site -COOH. O e. None of...
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