Selective media
Media that inhibit the growth of unwanted microorganisms and support the growth of the organism of interest by supplying nutrients and reducing competition are called Selective media. MacConkey agar is an example of this.
Many factors depend on the growth of the microorganisms.
1. Physical Requirements.
2. Chemical Requirements
There are many types of cultures are using. For bacterial culture we use.
For Virus and Phage culture we need a host cell in order to multiply.
Eukaryotic Cell Culture
Cell culture is the process by which cells are grown under controlled conditions, generally outside their natural environment. These conditions vary for each cell type.
There are different types of cultures for the growth of micro organism. For the growth of specific organism we use selctive medium to do the culture.
A culture technique that encourages the growth of specific microbes while inhibiting the growth of others...
41. The growth of microbes in an auenic culture on a solid medium may be limited by all of the following except Depletion of nutrients Accumulation of waste products. Lack of space or attachment sites Competition from other micrches. - -
While investigating the function of a specific growth factor receptor gene from humans, researchers found that two types of proteins are synthesized from this gene. A larger protein containing a membrane-spanning domain recognizes growth factors at the cell surface, stimulating a specific downstream signaling pathway. In contrast, a related, smaller protein is secreted from the cell and binds available growth factor circulating in the blood, thus inhibiting the downstream signaling pathway. Speculate on how the cell synthesizes these disparate proteins.
A batch culture has a constant specific growth rate of m = 0.2 hr-1. Due to the presence of a growth inhibitor, the cells also die at a constant (specific) death rate of 0.02 hr-1 at the same time. What is the doubling time of the culture?
QUESTION 33 Correctly match the following: Growth conditions implemented to favor the growth of specific bacterial A. fimbriae types over others B. capsule nutrient addition at mid to late log phase allows for further increase in cell C. pure culture yield D.complex medium - The exact composition of all chemical elements are known in this type of growth medium E. batch culture Important bacterial characteristic for biofilm formation F. Enrichment culture G.fed-batch culture H. minimal medium
Based on the growth data of a bacterial culture presented below, determine μ (specific growth rate). Time (Hour) X (Cell Concentration, Cells/L) 1 1,100,000 2 1,600,000 3 2,620,000 4 3,650,000 5 6,080,000 6 9,900,000 7 18,000,000 8 28,100,000 9 39,100,000 10 70,100,000 11 102,000,000
An exponentially growing culture has a specific growth rate constant, K = 2.4 day-1. What is the generation time? A test tube of fresh medium is inoculated with initial cell population of 6 x 105 cells/mL. Assuming no lag time, how long will it take for the culture to reach 8 x 109 cells/mL.
11.) A culture of bacteria in log phase increases from (Log1o ml) 8.0 to 8.6 in 1.5 h. What is specific growth rate (u) of this culture? What is the generation time (GT)? How many minut does it take for a doubling of cells? After 181 minutes, how many doublings have occurred? 12.) Finish labeling the following plot and determine the μ"ae and Ks of E.coli for glucose at 35°C. Us units of mM for concentration. Linweaver Burk plot E.coli...
Some consider fair access to health care a moral right, while others disagree. We have defined the term moral right as "a privilege to act in some specific, intentional manner or to obtain some specific benefit because one is a moral agent living in a community of moral agents under a shared moral standard." Is access to health care a moral right? Why or why not?
In order to count cells, the culture must be duted The technique o serialul precise and quantitative method alle e n al b e performed allow you to determine how many well per miller were the time the were made in overview knowvolume of culturedded to know Volume 0 sterile diluent aliquid that will not harm the cell The liquid in this tube is mixed and known volume of this culture is transferred to another known volume of sterile diluent...
Some consider fair access to health care a moral right, while others disagree. We have defined the term moral right as "a privilege to act in some specific, intentional manner or to obtain some specific benefit because one is a moral agent living in a community of moral agents under a shared moral standard." Discussion Prompt: Is access to health care a moral right? Why or why not?