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6. (20 points) You have created a green fluorescent protein (GFP) fusion to a protein that is normally secreted from yeast ce

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Ans) Strain B is wild-type because as stated in the question, the wild type protein is secretory and from the figure, it is shown the GFP protein is secreted out of the cell.

Strain A is defective in packaging of GFP protein in COPII vesicles for transport from ER to golgi complex. Hence, the GFP is accumulated in ER.

Strain C is defective in packaging of GFP into vesicle for release outside the cell. Hence, protein has accumulated in golgi complex.

Strain D is defective in transport of GFP from cis-golgi to trans-golgi and protein is seen accumulated in cis-golgi.

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