Question

What would be the consequence of primers that bound some bacterial species better than others? What is contained in a "master mix"? The master mix contains 8. Sometimes one can observe a "cloud" under the 100 bp fragment in a PCR gel (thus very small fragments of DNA). What produces this "cloud"? What type of charge does DNA possess? DNA possesses a

Answer 1

6- the primers which bind bacterial species better than others can be used to identify different bacterial species by knowing their genome and size

7- Master mix contain all components required for PCR to occur. It usually contains thermostable DNA polymerase , dNTPs , MgCl2 , specific buffer optimized for PCR and salts . Salts and Mg ions are required to optimum activity of taq polymerase.

8- Clouds are primer dimers . They are getting covered by too much DNA being loaded or due to high power giving them cloudy appearance.

9 - DNA possess negative charge .