Which of the following are newly acquired characteristics of E. coli cells successfully transformed with pGLO?...
Which of the following are newly acquired characteristics of E. coli cells successfully transformed with pGLO? select all that apply
arabinose production
green fluorescence under UV light
ampicillin resistance
lactose fermentation
Homework Answers
- arabinose production
- green fluorescence under UV light
- ampicillin resistance
Plasmid abbreviation is pGLO
Which denotes p - plasmid
G- Green flouresent undee UV light
L - resistance to beta lactamase and ampicillin
and arabinose production.
Arabinose production gene helps in green fluorescence production.
Lactose fermentation is not a acquired character. It is already a existing trait.
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Which of the following are newly acquired characteristics of
E. coli cells successfully transformed with pGLO?...
Suppose that you carried out a Bacterial transformation of E. coli HA101 with pGLO plasmid experiment...
Suppose that you carried out a Bacterial transformation of E. coli HA101 with pGLO plasmid experiment in the lab. During the experiment plates with bacteria were inoculated from +GLO and -GLO microfuge tubes (LB (-) plate, LB/amp (-) plate, LB/amp (+) plate, and LB/amp/ara (+) plate). 14) Explain what kinds of bacterial growth you will find on each of 4 plates after incubation ((LB (-) plate, LB/amp (-) plate, LB/amp (+) plate, and LB/amp/ara (+) plate): under normal light and...
hi Please help! Question 7 070.5 pts Which gene(s) on the PGLO plasmid are always turned...
hi Please help!
Question 7 070.5 pts Which gene(s) on the PGLO plasmid are always turned on (consitutively active)? Mark all that apply. amp' or bla (ampicillin resistance due to the expression of beta-lactamase) the enzymes for the catabolism of arabinose GFP (green fluorescent protein) Incorrect Question 8 0/0.5 pts Which gene(s) on the PGLO plasmid are turned on (expressed) when arabinose is added to the media? Mark all that apply. amp' or bla (ampicillin resistance due to expression of...
1. Fill in the table above with what you observe on your plates. 2. Bacterial transformation...
1. Fill in the table above with what you observe on your
plates.
2. Bacterial transformation occurred on which agar plate (s)?
What evidence do you have that the bacteria were transformed
here?
3. Which plates have glowing growth? Explain what causes
bacteria to glow.
II. Transformation of E. coli with Plasmid DNA (PGLO) 1. Three LB (Luria Broth) agar plates are obtained. The plates contain: • Plate A: LB-Agar/Ampicillin/Arabinose • Plate B: LB-Agar/Ampicillin • Plate C: LB-Agar 2. Three...
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1. Which plate(s) showed an unexpected result in the above experiment? 2. Provide the most likely...
1. Which plate(s) showed an unexpected result in the above
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2. Provide the most likely explanation for the unexpected
results.
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Answer the following questions that do not have an answer. Creating a graph is not necessary....
Answer the following questions that do not have an answer.
Creating a graph is not necessary. Thanks!!!
You introduced the pGlo vector into E. coli which carried the
GFP gene under the control of the arabinose
promoter. Based on what you know about the arabinose
promoter, when did you expect GFP to be expressed?
I expected the green fluorescence
protein to be expressed when arabinose was present.
What hypothesis was tested by the experiments you performed
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Bacterial cells, such as E. coli, are surrounded by a fluid plasma membrane and enclosed by a rigid cell wall that protects the cell from damage. You will recall that there is no nuclear envelope in prokaryotic cells, but rather a “nucleoid region” with a single chromosome that is continuous (circular), not linear, as in eukaryotes. All of the genes required for basic survival and reproduction are found in the main chromosome. In addition to the main chromosomes, some DNA...
hi Please help!
Question 7 070.5 pts Which gene(s) on the PGLO plasmid are always turned on (consitutively active)? Mark all that apply. amp' or bla (ampicillin resistance due to the expression of beta-lactamase) the enzymes for the catabolism of arabinose GFP (green fluorescent protein) Incorrect Question 8 0/0.5 pts Which gene(s) on the PGLO plasmid are turned on (expressed) when arabinose is added to the media? Mark all that apply. amp' or bla (ampicillin resistance due to expression of...
1. Fill in the table above with what you observe on your
plates.
2. Bacterial transformation occurred on which agar plate (s)?
What evidence do you have that the bacteria were transformed
here?
3. Which plates have glowing growth? Explain what causes
bacteria to glow.
II. Transformation of E. coli with Plasmid DNA (PGLO) 1. Three LB (Luria Broth) agar plates are obtained. The plates contain: • Plate A: LB-Agar/Ampicillin/Arabinose • Plate B: LB-Agar/Ampicillin • Plate C: LB-Agar 2. Three...
D7. Looking at your staging results, which phase of the cell cycle does the cell spend the most time in? interphase prophase metaphase anaphase telaphase cytokinesis 8. if we were using slides from a frog instead of an onion to do this lab, which cell type do you think would be best for us to view mitosis? frog skin frog embryo frog brain frog muscle D5. You want to use PGLO to produce red fluorescent protein (RFP) instead of green...
1. Which plate(s) showed an unexpected result in the above
experiment?
2. Provide the most likely explanation for the unexpected
results.
Table 2: Hypothetical results from transformation experiments performed in a pGLO plasmid added to cells? No No Type of agar plate Total # colonies # Green colonies under UV light LB LB + ampicillin LB + ampicillin LB + ampicillin+ arabinose 100 es
QUESTION 1 Match each item with its function in our transformation experiment. (5 points) - Makes cells permeable to plasmid DNA A. CaCl2 - Selects for the growth of transformed cells B. Ampicillin - Provides the GFP gene C. PGLO plasmid • Induces GFP gene expression D. Arabinose - Causes Green Fluorescent Protein to fluoresce E. UV Light QUESTION 2 To increase the efficiency of transformation, cells will be placed in a 42°C water bath for 50 seconds during which...
Answer the following questions that do not have an answer.
Creating a graph is not necessary. Thanks!!!
You introduced the pGlo vector into E. coli which carried the
GFP gene under the control of the arabinose
promoter. Based on what you know about the arabinose
promoter, when did you expect GFP to be expressed?
I expected the green fluorescence
protein to be expressed when arabinose was present.
What hypothesis was tested by the experiments you performed
after the pGlo vector was...
1. In a transformation experiment, E. coli uptake pRGLO plasmids that carry GFP gene. The transformed bacterial cells are grown on an ampicillin-treated agar plate. Which of the following is considered as a vector? GFP gene C E. coli cell c ampicillin-treated agar plate 0.5 points QUESTION 2 1. What is the function of bla gene? Break down the ampicillin Regulate the transcription of GFP gene Express the fluorescent trait in bacteria Control the uptake of DNA molecules in bacteria...
Bacterial cells, such as E. coli, are surrounded by a fluid plasma membrane and enclosed by a rigid cell wall that protects the cell from damage. You will recall that there is no nuclear envelope in prokaryotic cells, but rather a “nucleoid region” with a single chromosome that is continuous (circular), not linear, as in eukaryotes. All of the genes required for basic survival and reproduction are found in the main chromosome. In addition to the main chromosomes, some DNA...
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