Here we are isolating mitochondrial DNA from cheek cells. So for this we have taken 1.5 ml of swirled saline solution in micro centrifuge tube in swirled saline solution, cheek cells are there which is actual source of mitochondria DNA. So in step 5 this centrifuge tube is centrifuged which led to formation of pellet of cheek cells and clear solution.
Do that us why in step 6 supernatant is discarded and pellet is proceed in next step as this pellet is source of mitochondrial DNA.
After the above step , pellet is dissolved in lysis buffer . Lysis buffer lyse the cells and release the DNA ik to supernatant. That's what in step 16 after centrifugation, supernatant is collected as it contains mitochondrial DNA and lysed cells are discarded.
If we discard cell pellets at step 5 it will lead to loss of cells which contain mitchondrial DNA . If in step 1y we discard supernatant, then it will also lead to loss of mitochondrial DNA.
En (2 points) You isolated your mitochondrial DNA in Part I. In step 6, you discard...
I just need the answers to questions 2 and 3. My DNA ladder is in lane 2 with the yellow arrow pointing to it. Thanks! Part 2: Gel purification and ration Gel Slice and PCR Product Preparation modified from IBSci.com instructions for gel and PCR clean-up system A. Dissolving the Gel Slice 1. Following electrophoresis, excise DNA band from gel and place gel slice in a 1.5ml microcentrifuge tube. 1b. Use an analytical balance to weigh gel slice. Record weight...
For part 1 of this lab) I collected a soil sample from my campus Part 2) Tested bacteria initial viability Part 3) DNA extraction Part 4) DNA quantification by Nanodrop Part 5) Sample sequencing Part 6) PCR amplification Part 7) Gel electrophoresis Part 8) DNA sequence data analysis (sent sample to another lab) Directions for Part 3 DNA extraction are in the attached image QUESTIONS REGARDING PART 3 (DNA extraction) 1) What type of conclusions can be made from initially...
I need the answers for questions 2 and 3. My DNA ladder is in lane 2 marked by the yellow arrow. Thanks! Here is the only other info I have. Thanks! Part 2: Gel purification and on Gel Slice and PCR Product Preparin modified from TBSci.com instructions for gaan A. Dissolving the Gel Stie Following electrophores, eral DNA band from grand place glice microcentrifuge tube Ib. Use an analytical balance to weigh pelice Rec die 2. Add 500 balance to...
A student uses the same gel extraction and quantification protocols used in Lab 5, with the following exceptions: Only half of the 50µL pMD2 plasmid digestion reaction was loaded on the gel. The student did a 1:25 dilution of the extracted backbone during the quantification. The student measures the same absorbance (0.015). Using the protocol form the lab manual, what was the concentration (in ng/µL) of the plasmid stock concentration before performing the digestion reaction? (2pts) Gel extraction Materials PE...
Chapter 10 Review: Biotechnology Learning Objectives and Application Questions Learning objectives are identified for you to review your understanding of this topic. You are advised to reflect carefully on these objectives and check your own understanding to see if you have understood these essential concepts from this week’s reading. ANSWER THOROUGHLY all the application questions associated with each objective IN YOUR OWN WORDS. Learning Objective I: Evaluate the importance of biotechnology in modern societies (p. 228, 229) Application question #1...
help with questions 5 to 10 please PCB 3023L Lab #4 Protocol & Worksheet (30pt) You may work in your lab groups durine class. but all written answers must be completed individually in your own words. 1) Using the plasmid map for orientation 1 and the cDNA map as a guide, complete the plasmid map for orientation #2. (4pt) 612 1318 1 - EcoRi EcoRI Xbal ECORV -Xbal- 1662 +Bell EcoRI EcoRV Not FP -- Xhol X 2015 PRSP +...
1. Describe the functions of the following reagents in extraction of DNA from corn meal: proteinase K; guanidine HCI; SDS 2. Why is the ratio of the OD at 260 and 280 nm used to estimate DNA purity? 3. In one paragraph, summarize basic principles of PCR technique in your own words. List all the reagents you will need to perform a PCR experiment. Does this method tell you what genetic modifications were made? If yes, describe how you can...