Homework Answers
D is the right answer.
Lac operon is a negatucely regulated and inducible operon.
The repressor is synthesised by the regulatory gene. In presence of lactose, lactose binds to the repressor. The repressor becomes inactive and ut can not bind to the regulator region i.e. operator in DNA. That's why RNA polymerase proceeds further to structural genes and their expression occur.
When glucose is absent, then cAMP binds to the CAP. In this way CAP is in active form and binds to the regulatory DNA. This allows proper binding of RNA polymerase and structural gene expression occurs.
That's why right answer is D.
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See Hin Which of the following describes the Lacoperon in E.coli when lactose, but not glucose,...
Fill in the blanks in the following sentences by selecting from the drop-down menus. When E.coli...
Fill in the blanks in the following sentences by selecting from the drop-down menus. When E.coli cells are grown in the presence of high glucose and high lactose, the lac repressor is not bound to the lac operator, CAMP levels in the cell are high and CAP is mostly bound to the CAP binding site in the lac promoter. Under these conditions, transcription of the lac operon Select When E.coli cells are grown in the absence of glucose and presence...
If we placed a bacterium into a medium that contained neither lactose nor glucose (let's give...
If we placed a bacterium into a medium that contained neither lactose nor glucose (let's give them arabinose instead), what will be the level of transcription from the lac operon? O A OFF (fully repressed, but not activated) O B BASAL (neither repressed nor activated) O C HIGH (not repressed, but activated) O D OFF (both repressed and activated, but the repressor "wins") Under what conditions do we get maximum transcription from the lac operon? O A When both glucose...
Under which of the following conditions will transcription of the lac operon occur when the bacteria...
Under which of the following conditions will transcription of the lac operon occur when the bacteria are originally inoculated in a medium containing both glucose and lactose? a) Lactose present/glucose present b) Lactose present/glucose used up c) Lactose absent/glucose present d) Lactose absent/glucose used up e) a and b
1) Which of the following conditions is most likely to cause the lactose operon to be...
1) Which of the following conditions is most likely to cause the lactose operon to be transcribed? A) There is more glucose in the cell than lactose. B) There is glucose but no lactose in the cell. C) The cyclic AMP and lactose levels are both high within the cell. D) The CAMP level is high and the lactose level is low. 2) How does the transcription of structural genes in an inducible operon occur? A) It occurs continuously in...
The gene machine program shows you what happens when lactose is present in E. coli, and...
The gene machine program shows you what happens when lactose is present in E. coli, and how the lac operon is under negative control. However, the lac operon is also under positive control from a protein called CRP, eAMP Receptor Protein. The absence of the lac repressor is essential but not sufficient for effective transcription of the lac operon. RNA polymerase also depends on the presence of CRP. Like the lac repressor, which can bind to the DNA and lactose....
1) The lac operon is… a) Positive repressible b) Both negative inducible and positive repressible c)...
1) The lac operon is… a) Positive repressible b) Both negative inducible and positive repressible c) Positive inducible d) Negative inducible 2) An activator regulatory protein… a) Is involved in negative regulatory control b) Is a cis acting regulatory element c) Recruits RNA polymerase to the promoter d) Prevents RNA polymerase from binding the promoter 3) For the lac operon, the presence of the substrate (lactose) activates the repressor. True or False? a) true b) false 4) cAMP… a) Activates...
Consider an E. coli strain carrying a mutation in crp (encoding the catabolite activator protein) that...
Consider an E. coli strain carrying a mutation in crp (encoding the catabolite activator protein) that prevents CAP from binding CAMP. Predict whether the repressor and the activator is active (able to bind to DNA) or inactive (unable to bind to DNA), and the level of transcription in the following scenarios. Options for transcription levels include high, medium, leaky and off. a. When lactose is present but glucose is not, the repressor is and the activator is leading to levels...
3. The CAP activator protein and the Lac repressor both control the Lac operon (see Figure...
3. The CAP activator protein and the Lac repressor both control the Lac operon (see Figure 28-14). Fill out the table below with No expression, Low/Medium expression, or High expression to summarize when the Lac operon will be expressed in each of the three E. coli strains in the table. An example has been done for you. RNA polymerase- binding site (promoter) CAP binding site start site for RNA synthesis operator Lacz gene -80 -40 14080 nucleotide pairs Figure 08-14...
Chromosome Figure 6 Fiqure 7 R plasmid F plasmid integrated into host chromosome Sex pilus chromosome...
Chromosome Figure 6 Fiqure 7 R plasmid F plasmid integrated into host chromosome Sex pilus chromosome of recipient Figure 8 Double- Cel stranded Cytoplasmic ll ONA Integrated DNA Treat with electric current Recipient cell Fragments of donor DNA Transformed cell Phage Figure 9 Figure 10 Phage DNA enters the cell Bacterial host #1 앗 O E. coli F plasmid Gov Integration of F plasmid Bacterial DNA -Bacterial host #2 85 Replaced host DNA Bacterial DNA I Which diagram(s) depict conjugation?...
Quiz 10 1. (2 points) Which of the following conditions would lead to the highest levels of lac operon expression? a) High lactose, high glucose b) High lactose, low glucose c) Low lactose, high...
Quiz 10 1. (2 points) Which of the following conditions would lead to the highest levels of lac operon expression? a) High lactose, high glucose b) High lactose, low glucose c) Low lactose, high glucose d) Low lactose, low glucose e) None of the above would have any lac operon expression 2. (2 points) Which of the following is true concerning molecular genetics? a) tRNA carries amino acids into the nucleus in eukaryotic cells b) DNA polymerase moves towards the...
Fill in the blanks in the following sentences by selecting from the drop-down menus. When E.coli cells are grown in the presence of high glucose and high lactose, the lac repressor is not bound to the lac operator, CAMP levels in the cell are high and CAP is mostly bound to the CAP binding site in the lac promoter. Under these conditions, transcription of the lac operon Select When E.coli cells are grown in the absence of glucose and presence...
If we placed a bacterium into a medium that contained neither lactose nor glucose (let's give them arabinose instead), what will be the level of transcription from the lac operon? O A OFF (fully repressed, but not activated) O B BASAL (neither repressed nor activated) O C HIGH (not repressed, but activated) O D OFF (both repressed and activated, but the repressor "wins") Under what conditions do we get maximum transcription from the lac operon? O A When both glucose...
1) Which of the following conditions is most likely to cause the lactose operon to be transcribed? A) There is more glucose in the cell than lactose. B) There is glucose but no lactose in the cell. C) The cyclic AMP and lactose levels are both high within the cell. D) The CAMP level is high and the lactose level is low. 2) How does the transcription of structural genes in an inducible operon occur? A) It occurs continuously in...
The gene machine program shows you what happens when lactose is present in E. coli, and how the lac operon is under negative control. However, the lac operon is also under positive control from a protein called CRP, eAMP Receptor Protein. The absence of the lac repressor is essential but not sufficient for effective transcription of the lac operon. RNA polymerase also depends on the presence of CRP. Like the lac repressor, which can bind to the DNA and lactose....
Consider an E. coli strain carrying a mutation in crp (encoding the catabolite activator protein) that prevents CAP from binding CAMP. Predict whether the repressor and the activator is active (able to bind to DNA) or inactive (unable to bind to DNA), and the level of transcription in the following scenarios. Options for transcription levels include high, medium, leaky and off. a. When lactose is present but glucose is not, the repressor is and the activator is leading to levels...
3. The CAP activator protein and the Lac repressor both control the Lac operon (see Figure 28-14). Fill out the table below with No expression, Low/Medium expression, or High expression to summarize when the Lac operon will be expressed in each of the three E. coli strains in the table. An example has been done for you. RNA polymerase- binding site (promoter) CAP binding site start site for RNA synthesis operator Lacz gene -80 -40 14080 nucleotide pairs Figure 08-14...
Chromosome Figure 6 Fiqure 7 R plasmid F plasmid integrated into host chromosome Sex pilus chromosome of recipient Figure 8 Double- Cel stranded Cytoplasmic ll ONA Integrated DNA Treat with electric current Recipient cell Fragments of donor DNA Transformed cell Phage Figure 9 Figure 10 Phage DNA enters the cell Bacterial host #1 앗 O E. coli F plasmid Gov Integration of F plasmid Bacterial DNA -Bacterial host #2 85 Replaced host DNA Bacterial DNA I Which diagram(s) depict conjugation?...
Quiz 10 1. (2 points) Which of the following conditions would lead to the highest levels of lac operon expression? a) High lactose, high glucose b) High lactose, low glucose c) Low lactose, high glucose d) Low lactose, low glucose e) None of the above would have any lac operon expression 2. (2 points) Which of the following is true concerning molecular genetics? a) tRNA carries amino acids into the nucleus in eukaryotic cells b) DNA polymerase moves towards the...
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