Question

immunology:

these questions is for picture below.

A) where are two conclusions that you can draw from this figure?

b) what are the didferences that you would expect to see if you repeated this experiment for MHC II?

Peptides eluted from MCI inolecule 1 2 3 4 5 6 739 MN-V-G-P-O-K-N-E-N--coc- HN-S-G-P-R--A-1-0-1-coo- TH,N-V-G-P-S-G-K-Y-F-1-c00 HN-9-6-2---1-1-3-1-coo "Peptides eluted MN-S-Y-@---0-0-0-0-Coo from an MCI molecule from a -HN-DY-O-R-I---1-y-coo- different person HNS-Y-0-G-S-O-N-N-O-co- than above A-alanine Klysine R-arginine Eglutamic acid L-leucine $ *serine F = phenylalanine N = asparagine Ta threonine G = glycine P = proline V = valine Ha histidine Q-glutamine Y tyrosine Ta isoleucine,

For the second picture below:

- what length must the RSS spacer be to link the above V element to its appropriate J element?

- 7bp

-12bp

-23bp

-9bp

- none above

is it none?

3 question)

MHC diversity ultimately affect the (which one?)

-variety of BCRs thats expressed on mature b cells

-variety of peptides that presented on B and T cells

-# of APCs

-transmembrane domain

-rate of somatic hypermutation

Answer 1

1.A)There are 2 major differences in the patient for the peptides eluted from MHC 1 molecule and the other patient for the peptides eluted from MHC 1 molecule are

In the 1st patient with the peptides(MHC 1 molecule):It doesnt code for 2 Amino Acids :Threonine and Histidine

In the 2nd patient with the peptides(MHC 1 molecule):It doesnt code for 2 Amino Acids :Lysine and Phenylalanine

B) Differences that one would expect to see if you repeated this experiment for MHC II are

Generally MHC Class I present 8-10 amino acid peptides  and they are expressed on the surface of nearly all nucleated cells;

But MHC Class II presents 14-18 amino acid peptides primarily on Antigen presenting cells , where they present processed antigenic peptides to TH cells.

2.length must the RSS spacer be to link the above V element to its appropriate J element is None of the above

3.MHC diversity ultimately affect the variety of peptides that presented on B and T cells