Question

Hi! I am stuck on this question and already attempted it once and it said two of the four blanks was right but I am not sure which ones it was. (for reference I had genes, complementary, transcripts, and hybridization)

Question:

How have microarrays demonstrated that, although all cells of an organism have the same genome, some genes are expressed in almost all cells , whereas other genes show cell and tissue specific expression?

fill in the blanks:

microarrays identify which _____ are present in which cells by hybridization to different ______ probes contained in multiple _____. Different _____ patterns indicate that whereas some genes are expressed in almost all cells others show cell and tissue specific expression.

the options to fill in are: hybridization, spots, transcripts, random, genes, complementary

Answer 1

The answer should be : transcripts, complementary, genes and hybridization.

Microarrays identify which transcripts are present in which cells by hybridization to different complementary probes contained in multiple genes. Different hybridization patterns indicate that whereas some genes are expressed in almost all cells others show cell and tissue specific expression.

Reason : Microarrays are basically used for identification of transcripts, because same genome can produce different transcripts. Transcripts are the ones which are actually expressed differently in different cells. The probes will always be complementary to the sequence of interest, so that binding can take place. The different hybridization patterns will actually indicate the expression levels of genes in different cells.

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