Question

6) The following sequence of nucleotides is found in a single-stranded DNA template: ATT GCC AGA TCA TCC CAA TAG AT Assume that RNA polymerase proceeds along this template from left to right.

a) Which end of the DNA template is the 5' end and which end is 3'?

b) What is the sequence of the complimentary DNA strand?

c) Give the sequence and label the 5' and 3' ends of the mRNA copied from this template.

Answer 1

dear student to solve this question you should know that

During transcription,theRNA polymerase read the DNA template strand from 3 to 5 direction but mrna is formed in 5 to 3 direction.

a.since the dna template strand is read grom 3 to 5 direction ..so the left side will be 3end and right will be 5 end .

b.sequence of complimentary dna strand adinine is replaced by thymine or vise versa and cytosine by guanine or vise versa.

it will be

3-TAA CGG TCT AGT AGG GTT ATC TA-5

c.we disscussed above that mrna is formed in 5 to 3 direction.looking to contemplary dna strand in partr b ..and makin mrna throgh transcription. in formation of mrna adenine will be replaced by uracil.

5-AUU GCC AGA UCA UCC CAA UAG AU-3

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