For the PCR reaction you have to dilute the cDNA to 100 ng/µL. Your cDNA has...

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For the PCR reaction you have to dilute the cDNA to 100 ng/µL. Your cDNA has...

For the PCR reaction you have to dilute the cDNA to 100 ng/µL. Your cDNA has a concentration of 700 ng/µL. Taking 10 µL of this cDNA, by what factor to you have to dilute it and how much PCR water do you add?

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Answer 1

Answer #1

The stock cDNA concentration is 700 Ng/L and the final concentration will be 100 Ng/L .

So the solution have to be diluted 700/100 =7 times.

To make 10 L 7 fold diluted solution , you have to add 7 L of PCR water and (10-7)= 3 L of stock solution.

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Answer 2

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Homework Answers

Add Answer to: For the PCR reaction you have to dilute the cDNA to 100 ng/µL. Your cDNA has...

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For the PCR reaction you have to dilute the cDNA to 100 ng/µL. Your cDNA has...