Question

Hide product details - 315 630 Cross-Reacting Tran... NM_015017 (USP33), NM_006121 (KRT1), NM_015131 (WDR43), NM_201626 (USP33), NM_201624 (USP33), NM_173631 (ZNF547), NM_007110 (TEP1) Sequence Positions 173-198 Sequence Details Sense and antisense sequences Sequence Strand 5'rArArCrArUrGrGrCrArArGrGrArArGrArCrCrUrUrArArATT 3 5'rArArUrUrUrArArGrGrUrCrUrUrCrCrUrUrGrCrCrArUrGrUrUrGrA3
what is the guide strand

Answer 1

Ans: The antisense strand is the guide strand i.e the sequence represented by the negative sign is the guide strand and the sequence that is represented by positive sign is called as passenger strand. In the mechanism of RNA interference, a protein called dicer cleaves long dsRNA molecules to form short siRNA molecules which combines with proteins such as slicer and argonaute to form RISC complex where slicer removes the passenger strand. Guide strand along with the protein argonaute forms activated RISC complex which degrade the target mRNA molecule.

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