Question

Q2) complete the steps until step 12  

A ‘flow chart’ is a diagrammatic representation of the sequence of steps in a process. In science, a flow chart is usually intended to provide a brief outline of an experimental procedure. In the spaces below, drag and drop the specific steps into the boxes in the right order to concisely summarize the steps involved in the isolation of plasmid DNA from E. coli. The initial steps that were carried out for you have already been filled in. (1 mark)

(Step 1) Transform bacteria

                      

(Step 2) Allow bacteria to grow overnight

                       

(Step 3) Centrifuge (collect cells, discard supernatant)

                       

(Step 4) Resuspend cells in cell resuspension solution

         

- Centrifuge (discard pellet, collect supernatant)

- Add lysis buffer (NaOH, SDS)

- Add supernatant to spin column

- Wash spin column x2

- Add alkaline protease solution

- Centrifuge (discard flow-through fluid)

- Add neutralisation buffer

- Elute plasmid DNA with water

Answer 1

Step!: Transforom Bacteria Step2: Allow bacteria to grow overnight Step3 : Centrifuge collect cell, discard supernettent) Stepy : Resuspend cells in cell resuspension solution Steps: Add lysis buffer (NOOH, SDS) Steps: Add Alkaline Protease solution Stept: Add neutralisation buffer Step8 : Centrifuge (Discard pellet , Collect supernatent) Steps. Add Supernatent to spin Column Stepio: Centrifuge (Discard frow through fund) Step: Wash spin column x2 Step/2" Elute plasmid DNA with water,