Question

V Check Your Understanding 2.1 If a 4x objective is used, what is the total magnification of a microscope 2.2 Which objective lens will give you the greatest resolving power? 2.3 To move a specimen to the upper left-hand field of view, which way must one move the slide? 2.4 As magnification increases, does the amount of light increase or decrease? 2.5 with increased magnification, what happens to the field of view?! 2.6 As magnification increases, the depth of field is the distance between the objective lens and the specimen. CHAPTER 4 The Invisible World Understanding Microscopy and Cell Structure and Function s

Answer 1

2.1. Magnification is the product of magnification of objective lenses and ocular lenses. The magnification of ocular lens is always 10x. If the magnification of objective lenses is 4x then the total magnification will be 40x.

2.2. Maximum resolving power is obtained by using objective lens with highest magnification, 100x.

2.3. Lower right, we always have to move in the opposite direction if we want to move the specimen in some direction.

2.4. Decreases, the image becomes less sharp.

2.5. As the magnification increases, the field of view decreases.

2.6. As the magnification increases, the depth of field decreases.

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