Question

2. How is tutai -aagnification calculated? What is the total magnification of the low-power objective? 3. Define working distance 4. What happens to working distance as total magnification increases? Activity 3: Determining Field Diameter and Estimating the Size of Objects 1. Perform the following conversions. Am c.350 pm Based on the calculations that you made for your microscope, what is the field diameter for the a. scanning objective? b. low-power objective? c. high-power objective? d. oil-immersion objective? 2. um 3. If the field diameter for the 4X objective is 4.5 mm, then what is the field diameter when using the 10x objective? 4. Estimate the length of each of the following objects in μm. b. a. 400x 100×小 Activity 4: Perceiving Depth of Field 1. Define depth of field. 2. What happens to the depth of field as total magnification decreases?

Answer 1

ANSWER::

2.Total magnification is calculated by MULTIPLYING the power of EYEPIECE with the power of OBJECTIVE. The total magnification of low power objective is 10X.

3.Working distance is defined as the distance between the front edge of objective lens and the specimen surface.

4.As total magnification increases,the working distance DECREASES.

Activity 3

1.a.3mm=300 micrometre

b. 350micrometre=0.35 millimetre

c. 0.5mm=500micrometre

d. 1micrometre=0.001millimetre.

2.a.

b. 1.8mm(1800 micrometre)

c. 0.45mm(450 micrometre )

d. 2mm(2000 micrometre).

3.The field diameter when using 10X objective is 2mm.

Activity 4

1.Depth of field is the horizontal thickness below the objective lens of your microscope where the object is in sharp focus.

2.As total magnification decreases, the depth of field increases.