Homework Answers
| Name of objective | objective magnification | total magnification |
| scanning | 4X | 40X |
| low power | 10X | 100X |
| high power | 40X | 400X |
| oil immersion | 100X | 1000X |
total magnification is calculated by multiplying the objective lens power to the eyepiece that is 10X
Higher the OD, higher would be the concentration (Beer-Lambert law)
Solution B contains highest concentration
Solution C has lowest concentration
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Biology
ercise 1: Using a Light Microscope: 1. Fill out this table to calculate total magnification...
4. What is the total magnification you achieve using the following lenses? Total magnification Lens 10x...
4. What is the total magnification you achieve using the following lenses? Total magnification Lens 10x ocular lens 4X scanning objective lens 10X low-power objective lens 40X high-power objective lens 100X oil-immersion objective lens
3. In a bright-field compound microscope, starting from the illuminator, follow the pathway of light: a....
3. In a bright-field compound microscope, starting from the illuminator, follow the pathway of light: a. The first lens that provides magnification of the image is the c lor lens. b. The lens that further enlarges the image ten more times is the low power nel lens. 4. Open the iris diaphragm all the way while using the low power objective. What happens to the field of view and the quality of the image? Now close the iris diaphragm all...
2. How is tutai -aagnification calculated? What is the total magnification of the low-power objective? 3....
2. How is tutai -aagnification calculated? What is the total magnification of the low-power objective? 3. Define working distance 4. What happens to working distance as total magnification increases? Activity 3: Determining Field Diameter and Estimating the Size of Objects 1. Perform the following conversions. Am c.350 pm Based on the calculations that you made for your microscope, what is the field diameter for the a. scanning objective? b. low-power objective? c. high-power objective? d. oil-immersion objective? 2. um 3....
Lab Manual Total magnification is calculated by multiplying the magnification of the ocular lens (eyepiece) by...
Lab Manual Total magnification is calculated by multiplying the magnification of the ocular lens (eyepiece) by the magnification of the objective lens. The magnitication of a lens is imprinted on the lens casing. Total Magnification Observation: Total Magnification Calculate total magnification figures for your microscope, and record your findings in Table 2.3. Total Magnification Table 2.3 Total Magnification Objective Lens Ocular Lens Objective Scanning power (if present) Low power High power Oil immersion (if presents A microscope's field of view...
Please select the right answer! Thank you Figure 2: Microscope Calibration Table Objective Lens Total Mag...
Please select the right answer! Thank you
Figure 2: Microscope Calibration Table Objective Lens Total Mag Field Diameter (um) 4X 40X 4,500 10x 100X 1,800 40x 400X 450 100x 1000x 180 When an intestinal cell is viewed under the 40 X objective lens of the microscope its length is approximately 1/10th the field of view. Use the calibration table (fig.2) to determine the approximate length of this cell. 45 um O 0.45 um 180 um O 0.18 um
1. Name at least 3 ways you can control the amount of light that reaches the...
1. Name at least 3 ways you can control the amount of light that reaches the ocular lens. 2. Define resolution. What is the limit of resolution for the light microscope? 3. What effect does the wavelength of light used have on resolution? 4. If you view a bacterial smear under the oil immersion lens, but forget to add oil, will the image still be just about as clear as if you had added the oil. 5. Define parfocal. 6....
2 mocular microscope used to calculate total Because the image only one ocular to reach Both...
2 mocular microscope used to calculate total Because the image only one ocular to reach Both gors through eyes. 2 What is the to wat is the total magnification for all 45x, and 97 x objectives lensest e m microscope with 15 x oculars and 4%, 10% 4X, 10X, 5 Assunting that all other riables remain constant, explain wory light of shorter wavelength will produce a clearer image than light of longer wavelengths. 4 Why is wavelength the main limiting...
EXERCISE 1: USE AND CARE OF THE MICROSCOPE 13 0 Move the high-dry lens out of...
EXERCISE 1: USE AND CARE OF THE MICROSCOPE 13 0 Move the high-dry lens out of position, and place a drop of immersion oil on the area of the slide you are observing. Carefully click the oil immersion lens into position. It should now be immersed in the oil (FIGURE 1.5). Careful use of the fine-adjustment knob should bring the object into focus. Note the shape and size of the cells. Did the color of the cells change with the...
1. Fill out the following table by indicating which general technique (light microscopy (LM) or electron...
1. Fill out the following table by indicating which general technique (light microscopy (LM) or electron microscopy (EM]) could be used to observe each structure or phenomenon. Put "no" in the box if the technique could not be used. If light microscopy can be used, name one technique (bright-field, phase-contrast, fluorescence, etc.) that you think would be effective. You will find some useful information in Appendix 1 of this manual and Chapter 18 of your textbook. Structure or phenomenon Could...
EXERCISE 1:USE AND CARE OF THE MICROS D CARE OF THE MICROSCOPE 11. Alter use, remove...
EXERCISE 1:USE AND CARE OF THE MICROS D CARE OF THE MICROSCOPE 11. Alter use, remove the slide, wipe oil of it, put dist cover on the microscope, and return it to the designated area. 12. When a problem does with the microscope obtain help from the instructor. Do not use other microscope unless yours is declared out of action." FIGURE 14 Focusing the condenser. () Ching low power, lower the condenser til distinct circle of light is visible. Cester...
4. What is the total magnification you achieve using the following lenses? Total magnification Lens 10x ocular lens 4X scanning objective lens 10X low-power objective lens 40X high-power objective lens 100X oil-immersion objective lens
3. In a bright-field compound microscope, starting from the illuminator, follow the pathway of light: a. The first lens that provides magnification of the image is the c lor lens. b. The lens that further enlarges the image ten more times is the low power nel lens. 4. Open the iris diaphragm all the way while using the low power objective. What happens to the field of view and the quality of the image? Now close the iris diaphragm all...
2. How is tutai -aagnification calculated? What is the total magnification of the low-power objective? 3. Define working distance 4. What happens to working distance as total magnification increases? Activity 3: Determining Field Diameter and Estimating the Size of Objects 1. Perform the following conversions. Am c.350 pm Based on the calculations that you made for your microscope, what is the field diameter for the a. scanning objective? b. low-power objective? c. high-power objective? d. oil-immersion objective? 2. um 3....
Lab Manual Total magnification is calculated by multiplying the magnification of the ocular lens (eyepiece) by the magnification of the objective lens. The magnitication of a lens is imprinted on the lens casing. Total Magnification Observation: Total Magnification Calculate total magnification figures for your microscope, and record your findings in Table 2.3. Total Magnification Table 2.3 Total Magnification Objective Lens Ocular Lens Objective Scanning power (if present) Low power High power Oil immersion (if presents A microscope's field of view...
Please select the right answer! Thank you
Figure 2: Microscope Calibration Table Objective Lens Total Mag Field Diameter (um) 4X 40X 4,500 10x 100X 1,800 40x 400X 450 100x 1000x 180 When an intestinal cell is viewed under the 40 X objective lens of the microscope its length is approximately 1/10th the field of view. Use the calibration table (fig.2) to determine the approximate length of this cell. 45 um O 0.45 um 180 um O 0.18 um
2 mocular microscope used to calculate total Because the image only one ocular to reach Both gors through eyes. 2 What is the to wat is the total magnification for all 45x, and 97 x objectives lensest e m microscope with 15 x oculars and 4%, 10% 4X, 10X, 5 Assunting that all other riables remain constant, explain wory light of shorter wavelength will produce a clearer image than light of longer wavelengths. 4 Why is wavelength the main limiting...
EXERCISE 1: USE AND CARE OF THE MICROSCOPE 13 0 Move the high-dry lens out of position, and place a drop of immersion oil on the area of the slide you are observing. Carefully click the oil immersion lens into position. It should now be immersed in the oil (FIGURE 1.5). Careful use of the fine-adjustment knob should bring the object into focus. Note the shape and size of the cells. Did the color of the cells change with the...
1. Fill out the following table by indicating which general technique (light microscopy (LM) or electron microscopy (EM]) could be used to observe each structure or phenomenon. Put "no" in the box if the technique could not be used. If light microscopy can be used, name one technique (bright-field, phase-contrast, fluorescence, etc.) that you think would be effective. You will find some useful information in Appendix 1 of this manual and Chapter 18 of your textbook. Structure or phenomenon Could...
EXERCISE 1:USE AND CARE OF THE MICROS D CARE OF THE MICROSCOPE 11. Alter use, remove the slide, wipe oil of it, put dist cover on the microscope, and return it to the designated area. 12. When a problem does with the microscope obtain help from the instructor. Do not use other microscope unless yours is declared out of action." FIGURE 14 Focusing the condenser. () Ching low power, lower the condenser til distinct circle of light is visible. Cester...
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