Question

Lab Manual Total magnification is calculated by multiplying the magnification of the ocular lens (eyepiece) by the magnification of the objective lens. The magnitication of a lens is imprinted on the lens casing. Total Magnification Observation: Total Magnification Calculate total magnification figures for your microscope, and record your findings in Table 2.3. Total Magnification Table 2.3 Total Magnification Objective Lens Ocular Lens Objective Scanning power (if present) Low power High power Oil immersion (if presents A microscope's field of view is the circle visible through the lenses. The diameter of field is the length of the field from one edge to the other. Field of View Observation: Field of View Low-Power (10x) Diameter of Field 1. Place a clear, plastic ruler across the stage so that the edge of the ruler is visible as a horizontal line along the diameter of the low-power (not scanning) field. Be sure that you are looking at the millimeter side of the ruler 2 Estimate the number of millimeters, to tenths, that you see along the field: mm. (Hint: Start with one of the millimeter markers at the edge of the field.) Convert the figure to micrometers: um. This is the low-power diameter of field (LPD) for your microscope in micrometers. High-Power (40x) Diameter of Fleld 1. To compute the high-power diameter of field (HPD), substitute these data into the formula given: a. LPD low-power diameter of field (in micrometers) b. LPM low-power total magnification (from Table 2.3) c. HPM high-power total magnification (from Table 2.3) Example: If the diameter of field is about 2 mm, then the LPD is 2,000 um. Using the LPM and HPM values from Table 2.3, the HPD would be 500 am. 200 um. X. X. HPD LPD xPM HPM HPD (6oh) 22 Laboratory 2 Metric Measurement and Microscopy 20

Answer 1

Low power(10x) Diameter of Field :-

Table & 2 Oljedtive lens Total Haqrifie Objeative Ocular lens Seamng prar 40 X 10X 4X (ruernd Loa poa 100 X 10X 10X 400X High poraer 40X 10X Cil immursion 1000 X 10X 100 X (if pesent)

2. Estimate the number of millimeters , to lengths, that you see along the field : 18/10 = 1.8mm = 1800 micrometer. This is the LPD for your microscope.

High power ( 40x) Diameter of Field :-

1a. LPD = 1800 micrometer

b. LPM = 100x

c. HPM = 400x

Therefore, HPD = 450 micrometer

HPD LPD x LPM HPH HPD 18 00 X 450um 4 O