Biochemistry Question: What is the difference between using a monoclonal antibodies vs. using polyclonial antibodies when performing a Western Blot?
Biochemistry Question: What is the difference between using a monoclonal antibodies vs. using polyclonial antibodies when...
You are working on the protein α-catenin in C. elegans and have produced a monoclonal antibody that recognizes this protein. The normal protein identified by this antibody on a Western blot is 104kDa. You go on to identify a mutant that you think produces no protein at all (geneticist call such mutants “protein null” mutants) because Western blots performed using your antibody with protein from this mutant yield no signal. Later, however, another member of your research group sequences your...
Biochemistry Question You are studying the enzyme Holidase (53 kDa) and want to measure its kinetics (its reaction rates). Holidase catalyzes the conversion of the substrate SPBRK into the small molecule product VCTN. Neither SPBRK or VCTN are colored, which means you cannot measure those concentrations directly, but you do have a polyclonal Gt anti VCTN antibody as well as a monoclonal Ms anti VCTN antibody which you hope will be useful. What antibody technique that we covered in class...
Can you please explain what the right answer and why? 43. You are using an anti-mouse monoclonal antibody to a nonlinear conformational epitope in an ELISA. Which of the following is TRUE? This antibody should work well in a western blot from an SDS-PAGE gel This antibody can't be used in a western blot because it will be denatured by the residual SDS This antibody be useful in targeting a protein blotted from a native gel. This antibody should have...
As a continuation of the experiments in Question 1 you want to study the timing of the APC's activity more distinctly. To do this you treat your cells with MG132 - a proteasome inhibitor. When MG132 is added to cells proteins can still be ubiquitinated, but they are not degraded, because the proteasome is inhibited. In cells that are treated with MG132 you can monitor APC activity on Cyclin B using just the cyclin B a. antibody and a western...
Question 12 parts a,b,c Q12. Using the information provided below on ELISA, answer the following questions system to recognize an infectious and in Background While antibodies are used by the immune system to recognize scientists also use antibodies for a myriad of laboratory and the body, scientists also use antibodies for a agent in assays. If a scientist aims to identify a specific protein or mal y and clinical heterogeneous mixture such as a cell lysate or blood e such...
Using the book, write another paragraph or two: write 170 words: Q: Compare the assumptions of physician-centered and collaborative communication. How is the caregiver’s role different in each model? How is the patient’s role different? Answer: Physical-centered communication involves the specialists taking control of the conversation. They decide on the topics of discussion and when to end the process. The patient responds to the issues raised by the caregiver and acts accordingly. On the other hand, Collaborative communication involves a...