Homework Answers
Protein X : Microfilament destabilizer.
This protein is a probable microfilament destabilizer as we can see the effective cellular concentration of microfilament falls in presence of this protein. The prior concentration of microfilament was 10uM. The concentration is reduced down to 1uM due to actin disassembly mediated by Protein X.
Protein Y: Microfilament stabilizer.
This protein is a probable microfilament stabilizer which helps in actin assembly during actin polymerization. This increases the effective concentration to 100uM.
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3. As a graduate student, your advisor asks you to determine the role of protein X...
16. You have cloned a centromere from yeast. You have also identified three genes, X, Y...
16. You have cloned a centromere from yeast. You have also
identified three genes, X, Y and Z that code for proteins that are
a subset of the kinetochore proteins that are bound to the
centromere. None of your three genes is essential but each does
contribute to the overall efficiency of chromosomal segregation.
You raise antibodies to each of your three proteins. You also
contstruct four strains, a wild-type strain, a strain in which gene
X is deleted (XD),...
1) You are a studying the prog ression of cells through the cell cycle. You are particularly interested in the role of...
1) You are a studying the prog ression of cells through the cell cycle. You are particularly interested in the role of the APC, which in a ubiquitin E3 ligase, in the control of the metaphase cyclin (cyclin B) and the timing of events. To look at this you start by synchronizing your cells in G1 and examining the levels of Cyclin B over time. The APC usually works on cyclin B during anaphase. RBX57 is a substrate for Cylcia...
You are studying X rightarrow Y You collect data to make the plot shown below: a)...
You are studying X rightarrow Y You collect data to make the plot shown below: a) Based upon the graph, what happens to the rate when you double your concentration of X? b) What is the rate law for the reaction? (rate law includes three parts: "rate =", proportionality constant, and concentration dependence) c) What units should your proportionality constant (k) have?
You are part of a student research group investigating the potential role of specific cytokeletal elements...
You are part of a student research group investigating the potential role of specific cytokeletal elements (microtubules and microfilaments) and motor proteins (kinesin, dynein and myosin) in SARS – CoV2 infection. You and your colleagues are submitting a grant proposal based on experiments you will perform to determine which of these cytoskeletal structures and proteins is/are essential for viral entry, replication and release of progeny (new virus particles). In this essay, clearly describe the experiments you propose, the cultured cells...
You are interested in studying the expression of Neurogenesis-2 (NG-2), a protein that may have a...
You are interested in studying the expression of Neurogenesis-2 (NG-2), a protein that may have a role in neurogenesis in the brain. You know that neurogenesis slows/stops as mice age, so you hypothesize that it will be highly expressed in young mice compared to old mice. (NG-2 is a fake protein used for this test question). Please note that you are being asked to analyze NG-2 at the PROTEIN level. Select appropriate techniques and technologies. (20 pts total). a) Describe...
You inject a mouse with a protein antigen X. This is the first time the mouse...
You inject a mouse with a protein antigen X. This is the first time the mouse has been exposed to X. In the course of clearing X from their body the mouse will launch an immune response to antigen X. Over the course of the immune response antigen X will encounter naïve, effector and memory lymphocytes (B- and T-cells). There are distinct differences between the function and role of each type (naïve, effector and memory). Differentiate between naïve, effector and memory...
3. Utilizing the data below to calculate the absorbances of your dilutions from part 2. Known...
3. Utilizing the data below to calculate the absorbances of your dilutions from part 2. Known Concentration % Transmittance 0.20 0 0.18 13 0.16 0.14 16 20.1 26 0.12 4. Type your concentrations into the data table below. Generate a graph of your dilution data from part 2 above. Absorbance Solution Concentration OM (blank) 4 When finished, start calculating the data below, based on your dilution data from part 2. Perform the best linear regression using Graphing calculator or Excel...
1)You inject a mouse with a protein antigen X. This is the first time the mouse...
1)You inject a mouse with a protein antigen X. This is the first time the mouse has been exposed to X. In the course of clearing X from their body the mouse will launch an immune response to antigen X. Over the course of the immune response antigen X will encounter naïve, effector and memory lymphocytes (B- and T-cells). There are distinct differences between the function and role of each type (naïve, effector and memory).Differentiate between naïve, effector and memory lymphocytes. Focus...
On your first day on the job, your boss asks you to conduct a hypothesis test...
On your first day on the job, your boss asks you to conduct a hypothesis test about the mean dwell time of a new type of UAV. Before you arrived, an experiment was conducted on ? = 5 UAVs (all of the new type) resulting in a sample mean dwell time of ?1 = 10.4 h????. The goal is to conclusively demonstrate, if possible, that the data supports the manufacturer’s claim that the mean dwell time is greater than 10...
5. (9pts) You are studying an ER membrane protein, YFG1 (outlined in yellow), with three transmembrane...
5. (9pts) You are studying an ER membrane protein, YFG1 (outlined in yellow), with three transmembrane domains (rectangles A, B & C at the amino acid positions according to the following map, connected by soluble domains (wavy lines): N-term 10 10 Com Amino acid position (not to scale) 30 60 80 140 160 310 ABCI YFG1 alkaline phosphatase To determine how the protein is arranged in the ER membrane, you construct a series of shortened YFG1 proteins that are connected...
16. You have cloned a centromere from yeast. You have also
identified three genes, X, Y and Z that code for proteins that are
a subset of the kinetochore proteins that are bound to the
centromere. None of your three genes is essential but each does
contribute to the overall efficiency of chromosomal segregation.
You raise antibodies to each of your three proteins. You also
contstruct four strains, a wild-type strain, a strain in which gene
X is deleted (XD),...
1) You are a studying the prog ression of cells through the cell cycle. You are particularly interested in the role of the APC, which in a ubiquitin E3 ligase, in the control of the metaphase cyclin (cyclin B) and the timing of events. To look at this you start by synchronizing your cells in G1 and examining the levels of Cyclin B over time. The APC usually works on cyclin B during anaphase. RBX57 is a substrate for Cylcia...
You are studying X rightarrow Y You collect data to make the plot shown below: a) Based upon the graph, what happens to the rate when you double your concentration of X? b) What is the rate law for the reaction? (rate law includes three parts: "rate =", proportionality constant, and concentration dependence) c) What units should your proportionality constant (k) have?
3. Utilizing the data below to calculate the absorbances of your dilutions from part 2. Known Concentration % Transmittance 0.20 0 0.18 13 0.16 0.14 16 20.1 26 0.12 4. Type your concentrations into the data table below. Generate a graph of your dilution data from part 2 above. Absorbance Solution Concentration OM (blank) 4 When finished, start calculating the data below, based on your dilution data from part 2. Perform the best linear regression using Graphing calculator or Excel...
5. (9pts) You are studying an ER membrane protein, YFG1 (outlined in yellow), with three transmembrane domains (rectangles A, B & C at the amino acid positions according to the following map, connected by soluble domains (wavy lines): N-term 10 10 Com Amino acid position (not to scale) 30 60 80 140 160 310 ABCI YFG1 alkaline phosphatase To determine how the protein is arranged in the ER membrane, you construct a series of shortened YFG1 proteins that are connected...
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