Question

All of 20 hing Med ia 1 The incide d together by chemical beads to form long chains, in the same way that ar e made of long chains of simple molecules Unlike carboidrates, however, protein molecules fold pinc h pes are for their higical function. The folded structure of a protein depends on the sequence of the amino acid building block that forms the polymer chain. A folded protein is shown on the side of the diagram below. The polymer chain of mine weite). structur i ng of loops and is in a fixed relationship to each other. Denaturation Normal protein Densured protein Renaturation Denaturation causes a protein molecule to undergo a radical change in shape. With the exception of providing energy, a denatured protein is no longer able to carry out its biological function. There are many ways to denature a protein. Heating is one method. Every time we cook an ere, the protein og albumin in the white decatures and becomes a white solid. The skin that forms when we heat milk is the protein casein solidifying and separating from the liquid. Exposure to chemicals such acid may also denature proteins. Sometimes the protein can refold, reversing the process, but that is not the case in our experiment. In the first part of this experiment, we will denature casein, the protein in milk, using acetic acid. We will observe that the denatured protein becomes a solid, allowing us to separate it from the liquid components of the milk or whey. We can then measure the mass of the protein and determine the protein in the milk sample. We will compare our result with what we expect based on the food label. All whole animal and plant-based foods contain some protein. While protein nutrient is most concentrated in the meat nuts and dairy food groups, all whole foods in the Food Pyramid contain some protein. Protein content is measured by a chemical test called the biuret test. In the second part of this experiment, we will measure the protein content of several foods by this method. Before a protein can be used by the body, the long chain of amino acids most be broken down by digestion. The process is started in the stomach through the action of an enzyme called pepsin. When the protein is de graded, what remains is a solution of amino acids. which can enter the circulatory system by absorption through the membrane of the small intestine. Some of the amino acids are metabolized in the body to produce energy. When this occurs, they yield 4 calories/gram of food energy. Amino acids are also rassembled to form the enzymes and proteins our bodies require to function. Without essential amino acids obtained from food, our odies would not be able to make the proteins required to sustain life.

can you just answer 1,3,4 then ?

Answer 1

Question 1:

The purpose of this experiment is to denature protein using acetic acid. After heating milk when acetic acid is added to that milk protein denatures and becomes solid ( Coagulates).
This Solid mass will be separated and tested for % Protein content.
The next experiment will be to test the presence of protein in a certain sample by biuret test.

Question 3:

Amino Acid	Source 1	Source 2	Source 3
Isoleucine	Eggs	Cheese	Seaweed
Histidine	Fish	Meat	Rice, Wheat
Leucine	Chicken	Tofu	Legumes
Lysine	Fish ( Cod and Sardines)	Lamb	Peas and Beans
Methionine	Eggs	Whitefish	Sesame seeds, Brazil nut
Phenylalanine	Beef	Yogurt, Cheese	Soybean Flour
Threonine	Poultry	Cottage Cheese	Black Turtle bean
Tryptophan	Salmon	Milk	Spinach, Nuts
Valine	Meat	Cheese	Mushroom, Peanuts

Question 4:

Non-essential amino acids can be prepared by the body while the essential amino acid cannot be prepared by the body. There are nine essential amino acid which can be found in a variety of food sources.

Deficiency of essential amino acid can lead to low immunity, digestive problems, depression, etc.