Question

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4. In the Kirby-Bauer susceptibility testing method: (5) a. Why must the standard suspension of organism be inoculated within 15 minutes? How will it affect the zone size?

Answer 1

The Answer The standard suspension of organism is necessary to be innoculated within 15 min in order to prevent overgrowth, as some organismii would definitely continue to grow, which will result in a different concentrations. Some of them would become too old, which would result in the accumulation of metabolites in the medium even before innoculation. The horonous nature of the innoculant is also lost after 15 min... B This tine span plays a nebal role o the development of inhibition zones e 15 min time span is not followed cesult in this may rest a incorrect concetto #smaller) er hibition des transitionat Zones, irrespective of the antibiotic susceptibility

The Kirby-Bauer antibiotic susceptibility test assay is a popular method to study the effect of antibiotic on the different groups of microbes and identify the susceptible groups.